基于线粒体Cyt b基因的皖南山区温州光唇鱼种群遗传结构

doi:10.11924/j.issn.1000-6850.casb16110005

中国农学通报 ›› 2017, Vol. 33 ›› Issue (32): 121-126.doi: 10.11924/j.issn.1000-6850.casb16110005

所属专题：生物技术；水产渔业

基于线粒体Cyt b基因的皖南山区温州光唇鱼种群遗传结构

胡玉婷,江河,段国庆,凌俊

安徽省农业科学院水产研究所,安徽省农业科学院水产研究所,安徽省农业科学院水产研究所,安徽省农业科学院水产研究所

收稿日期:2016-11-01 修回日期:2017-11-07 接受日期:2016-12-15 出版日期:2017-11-27 发布日期:2017-11-27
通讯作者: 江河
基金资助:
安徽省现代农业产业技术体系专项“安徽省水产产业技术体系”（皖农科[2016]84 号）；安徽省农科院学科建设面上项目(15A0508)

Population Genetic Structure of Acrossocheilus wenchowensis in Mountainous Area of Southern Anhui Province Based on Mitochondrial Cyt b gene

Received:2016-11-01 Revised:2017-11-07 Accepted:2016-12-15 Online:2017-11-27 Published:2017-11-27

摘要/Abstract

摘要： 为探讨皖南山区温州光唇鱼的种群遗传结构,采用线粒体细胞色素b基因(Cyt b)对该区5个野生种群(祁门、黟县、休宁、旌德和宁国)进行群体遗传变异分析。131个样本的Cyt b基因(1141 bp)中共检出38个变异位点(变异率3.33%)、14种单倍型。序列碱基的平均含量分别为A(28.2%)、C(29.7%)、T(27.0%)、G(15.1%),A T 含量(55.2%)明显大于 G C 含量(44.8%)。5个地理种群的单倍型多样性(0.0000-0.6799)和核苷酸多样性(0.0000-0.00759)普遍较低。群体分化指数(FST: 0.2916-0.9782)和AMOVA 分析中高达52.74%的遗传变异来自地理种群间,说明温州光唇鱼地理种群间已产生显著遗传分化；但不同水系的种群间没有显著遗传差异。群体间系统进化树显示：5个群体聚为两大进化枝(祁门和旌德种群为一枝,其余种群为另一枝)。温州光唇鱼的这种种群遗传结构与地理隔离及其生态习性相关。

关键词: 生物腐植酸, 生物腐植酸, 土壤碳组分, 土壤水溶性有机碳, 微生物生物量碳

Abstract: In order to study the population genetic structure of Acrossocheilus wenchowensis in mountainous area of southern Anhui Province, mitochondrial cytochromeb (Cytb ) gene from 5 populations (Qimen, Yixian, Xiuning, Jingde and Ningguo) with 131 individuals of A. wenchowensis were amplified and sequenced bidirectionally. Finally 14 haplotypes were identified and 38 mutation sites were detected. The average contents of T, C, A and G were 27.0%, 29.7%, 28.2% and 15.1% respectively, A+T content (55.2%) was higher than G+ C content (44.8%). The haplotype diversity (0.0000-0.6799) and nucleotide diversity (0.0000-0.00759) of the 5 populations were all low. The genetic differentiation index (F ST) of populations ranged from 0.2916 to 0.9782. Analysis of molecular variance (AMOVA) indicated that there was 52.74% of variance among populations, suggesting that populations had significant genetic differentiation. But there was no obvious genetic difference among populations from different rivers. The molecular phylogenetic tree (NJ- tree) showed that the 5 populations could be divided into two groups, with Qimen and Jingde populations clustered into group 1 and the other three populations clustered into group 2. The results were related to geographic isolation and ecological habit of A. wenchowensis.

中图分类号:

S917. 4

胡玉婷,江河,段国庆,凌俊. 基于线粒体Cyt b基因的皖南山区温州光唇鱼种群遗传结构[J]. 中国农学通报, 2017, 33(32): 121-126.

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基于线粒体Cyt b基因的皖南山区温州光唇鱼种群遗传结构

Population Genetic Structure of Acrossocheilus wenchowensis in Mountainous Area of Southern Anhui Province Based on Mitochondrial Cyt b gene

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