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中国农学通报 ›› 2017, Vol. 33 ›› Issue (32): 113-120.doi: 10.11924/j.issn.1000-6850.casb17050125

所属专题: 生物技术 水产渔业

• 水产 渔业 • 上一篇    下一篇

金线鱼鱼皮抗冻蛋白包埋工艺的研究

张怡,吴珊,邓昌俊,沈瑾烨,陈怡,傅维擎,曾红亮,郑宝东   

  1. 福建农林大学食品科学学院,福建福州,福建农林大学食品科学学院,福建福州;宁德师范学院,福建农林大学食品科学学院,福建福州,福建农林大学生命科学学院,福建农林大学食品科学学院,福建福州,福建农林大学食品科学学院,福建福州,福建农林大学食品科学学院,福建福州,福建农林大学食品科学学院,福建福州
  • 收稿日期:2017-05-27 修回日期:2017-10-31 接受日期:2017-11-03 出版日期:2017-11-27 发布日期:2017-11-27
  • 通讯作者: 张怡
  • 基金资助:
    福建农林大学国际科技合作与交流资助项目(KXGH17001);福建省高等学校新世纪优秀人才支持计划“海洋功能蛋白因子的制备及其在 高品质鱼糜制品加工中的应用”(JA14094);福建省发改委产业技术联合创新专项项目“海洋天然活性抗冻肽的制备及其在高品质鱼糜制品中的应 用”(闽财指[2015]779 号);福建省科技创新领军人才(KRC16002A);福建农林大学横向科技项目“海洋天然抗冻蛋白的制备及在鱼糜制品中的应用 项目立项可行性研究”(KH1600260)。

Optimization on Embedding of Antifreeze Proteins from Skin of Nemipterus virgatus

吴珊,,沈瑾烨,陈怡,傅维擎,曾红亮 and 郑宝东   

  • Received:2017-05-27 Revised:2017-10-31 Accepted:2017-11-03 Online:2017-11-27 Published:2017-11-27

摘要: 为了提高金线鱼鱼皮抗冻蛋白的热稳定性,本文采用多孔变性淀粉对金线鱼鱼皮抗冻蛋白进行包埋,对其包埋工艺和热稳定性进行研究。本文分别研究多孔变性淀粉壁芯材比、包埋温度和包埋时间等对金线鱼鱼皮抗冻蛋白包埋率的影响,并对其热滞活性进行了测定。结果表明,多孔变性淀粉包埋金线鱼鱼皮抗冻蛋白的最佳工艺条件为:壁芯材比为30:1、包埋温度为39℃、包埋时间为120min,在此条件下,包埋率可达85.12±1.10%;各因素对抗冻蛋白包埋率的影响程度大小依次为:包埋温度>壁芯材比>包埋时间;热稳定性分析表明,多孔变性淀粉对抗冻蛋白起到了保护作用,提高了抗冻蛋白的热稳定性。

关键词: 农民工, 农民工, 市民化, 评价指标体系

Abstract: In order to improve the thermal stability of the anti-freeze skin proteins from Nemipterus virgatus, the porous modified starch was used to embed the anti-freeze proteins from fish skin in this paper, and then their embedding technology and thermal stability were studied. The effects of the ratio of wall material and core materials (W/C ratio), embedding temperature and time on the embedding rate of antifreeze proteins from fish skin were investigated respectively. Moreover, the thermal hysteresis activity was determined. The results showed that the optimum embedding conditions of AFP by the porous modified starch were as following: W/C ratio of 30:1, embedding temperature of 39 ℃, embedding time of 120 min. Under these conditions, the embedding rate could be up to 85.12 ± 1.10%. The effect of variables on the embedding rate of AFP are in the order: embedding temperature > W/C ratio > embedding time. The thermal stability analysis showed that the AFP was protected by the porous modified starch effectively, which improved the thermal stability of AFP of fish skin from Nemipterus virgatus.

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