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中国农学通报

中国农学通报 ›› 2005, Vol. 21 ›› Issue (5): 327-327.

所属专题： 棉花

• 目次 • 上一篇    下一篇

新型选择性培养基检测土壤棉花黄萎病菌效果初报

贾 涛,杨家荣   

  • 出版日期:2005-05-05 发布日期:2005-05-05

A New Selective Medium to Be Used to Simply and Quick Isolate Verticillium dahliae in Cotton from Naturally Infested Soil

Jia Tao, Yang Jiarong   

  • Online:2005-05-05 Published:2005-05-05

摘要： 介绍了一种用于检测土壤棉花黄萎病菌的选择性培养基。该培养基成份为：鲜棉叶100g，蔗糖5g，琼脂17~20g，40%PCNB0.3g，氯霉素、链霉素和青霉素各0.05g，水1000ml，pH6.4~7.0。该培养基与其它常见分离培养基相比，成份简单、操作简便，检测率显著，抑菌效果好，病菌在其上生长快、菌落典型，是一种较为理想的选择性分离培养基。

Abstract: A new selective medium to be used to simply and quick isolate Verticillium dahliae in cotton from naturally infested soil was introduced. The medium consisted of following ingredients per liter distilled water: leaves cotton 100g, sugar 5g, agar 11~20g, 40% PCNB 0.3g. chloramphenicol 0.05g, streptomycin 0.05g and penicillin 0.05g, pH adjusted to 6.4~7.0. The medium is simple and quick than old that. The microscclerotia V. dahliae from naturally infested soil could be effectively isolated while it was rarely infected by other pathogens. The colonies of V. dahliae on the medium were large black-colored.