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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (9): 175-179.

• 生物技术科学 • 上一篇    下一篇

氯嘧磺隆降解细菌的分离鉴定及其降解特性

纪明山 颜克成 刘周成 冯俊义 黄均艳   

  • 收稿日期:2011-11-07 修回日期:2012-01-15 出版日期:2012-03-25 发布日期:2012-03-25

Isolation, Identification of Chlorimuron-ethyl Degradation Bacteria and Its Degradation Characteristics

  • Received:2011-11-07 Revised:2012-01-15 Online:2012-03-25 Published:2012-03-25

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摘要：

为了解决大豆田除草剂氯嘧磺隆在土壤中残留时间长的问题，为氯嘧磺隆污染的土壤的生物修复提供菌源，利用富集培养技术，从多年使用氯嘧磺隆的土壤中分离得到1株能以氯嘧磺隆作为唯一氮源生长的细菌，命名为SN10菌株。通过对该菌株的生理生化鉴定和16S rDNA序列分析，初步鉴定菌株SN10为蜡样芽孢杆菌(Bacillus cereus)。以SN10菌体生长量、氯嘧磺隆降解率为指标，研究了（温度、pH、接菌量、培养时间）对SN10生长量和降解能力的影响。结果表明，SN10最佳生长量和降解条件为28℃，pH为7，培养时间4天，接菌量为7%，在此条件下，SN10菌株对氯嘧磺隆的降解率达到87.2%。通过氯嘧磺隆降解菌的修复效果评价试验，得出加菌加药处理组的玉米种子的发芽率、株高菌均高于仅加药的处理。SN10对土壤中氯嘧磺隆的降解效果明显。

关键词: 畜禽养殖, 畜禽养殖, 总量控制, 允许总量, 氮磷承载力

Abstract:

In order to solve the problem of soybean herbicide chlorimuron-ethy residues in soil for long time. To provide actinomyces source for bioremediation of soil contaminated by chlorimuron-ethy. A bacteria which was able to degrade chlorimuron-ethyl and could grow using chlorimuron-ethyl as the sole nitrogen, was isolated from the soil of applying with chlorimuron-ethyl for many years by enrichment culture, which was named SN10. Based on physiological and biochemical characteristics and 16S rRNA sequence, the strain was identified preliminarily as Bacillus cereus. The influencing factors (temperature, pH, inoculation amount and incubation time) were studied with bacteria growth mass and degradation ration as indexes. The results showed that the optimal degradation ratio occurred at the condition of 28℃, pH 7, inoculation amount of 7%, incubation time 4 days. Under these conditions, 81.2% chlorimuron-ethyl had been degraded. By chlorimuron-ethyl degradation bacteria evaluation of the repair test, bacteria and drug treatment of maize seed germination and plant height were higher than only drug treatment, SN10 degraded obviously chlorimuron-ethyl in soil.