欢迎访问《中国农学通报》,

中国农学通报 ›› 2012, Vol. 28 ›› Issue (33): 12-17.

所属专题: 水稻

• 农学 农业基础科学 • 上一篇    下一篇

离心超重力诱发水稻变异效应初报

陈建伟 罗文永 王丰 黄慧君 肖昕 柳武革 廖亦龙 朱满山 付崇允 李金华 刘振荣   

  • 收稿日期:2012-03-16 修回日期:2012-05-14 出版日期:2012-11-25 发布日期:2012-11-25
  • 基金资助:

    广东省自然科学基金项目“水稻离心诱变育种技术及其效应研究”

Study on Rice Mutation induced by Centrifugal Force

  • Received:2012-03-16 Revised:2012-05-14 Online:2012-11-25 Published:2012-11-25

摘要:

为了研究离心超重力是否对水稻产生诱变作用,通过采用9个不同的离心超重力和处理时间组合处理水稻品种矮秀占的萌动种子,并对处理后代进行研究。结果表明,随着处理的离心超重力加大和处理时间延长,发芽率下降,M2代的变异频率上升。处理效果以超重力为12000×g,处理时间60 min最好。处理M1代未发现明显的表型变异,M2代发生多样性变异,主要表现在茎秆、叶片、生育期、穗部性状、籽粒、稻米外观和直链淀粉含量,并可以稳定遗传。说明离心超重力不仅对水稻处理当代植株的生长发育产生抑制作用,而且可以诱发水稻产生明显的可遗传变异,可以作为一种新的诱变育种手段。

关键词: CaCl2盐溶液, CaCl2盐溶液

Abstract:

The germinating seeds of the rice cultivar Aixiuzhan were treated with 9 different combinations of centrifugal forces and treatment times and cultivated for generations in order to see if the centrifugal force had induction to rice. The results showed that, their germination percentage was decreased, and the rate of induced mutation increased in their M2 generation while the centrifugal force increased, and the treatment duration extended. The highest rate of the induced mutation was presented under the combination of 12000×g centrifugal force and 60 minutes of treatment time. There was no visible mutation founded in M1 generation, but there were varied inheritable mutations founded in M2 generation, and that were hereditary stably. The variations mainly happened on the stem, leaf, growth duration, panicle, grain, appearance and amylose content. It indicated that, the centrifugal force could inhibit the germination of rice seeds in M1, and did also induce inheritable mutations in rice seeds which could be used as a new induced mutation technique for rice breeding.