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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (23): 32-35.doi: 10.11924/j.issn.1000-6850.2012-1056

所属专题： 生物技术； 畜牧兽医

• 畜牧 动物医学 蚕 蜂 • 上一篇    下一篇

猪耳缘成纤维细胞的体外培养

薄旭 林浩 刘云海 郭勇 倪和民   

  • 收稿日期:2012-03-23 修回日期:2012-04-23 出版日期:2012-08-15 发布日期:2012-08-15
  • 基金资助:

    国家自然基金面上资助项目（31072185），北京市自然基金重点项目（5091002）

The in vitro Culture of Pig Ear Skin Fibroblast

  • Received:2012-03-23 Revised:2012-04-23 Online:2012-08-15 Published:2012-08-15

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814

摘要：

为了建立猪耳缘成纤维细胞培养体系，为猪核移植实验做准备。主要采用组织块法进行猪耳缘成纤维细胞的培养、传代、冷冻和复苏，观察细胞的形态、生长和贴壁能力。结果显示：组织块培养法培养的细胞，生长较快，呈放射状，体外能正常传代。冷冻和复苏后，细胞能够正常的贴壁、增殖，经胰酶消化后，为核移植实验提供良好的细胞核供体。说明组织块培养方法是简单有效的获得猪耳缘成纤维细胞的方法，获得的细胞可以在体外至少传4代，能够为猪核移植胚胎提供供体细胞。

关键词: 相关分析, 相关分析

Abstract:

A method was described for isolation and culture of pig ear skin fibroblast cells defined for porcine somatic nuclear transfe. The experiment was carried out with pig ear skin fibroblasts for primary culture, routine subculture, freezing, and recovery procedure by tissue-pieced method. Those in vitro cultured cells were analyzed by morphology, growth trends and adherent ability. The results showed that pig ear skin fibroblasts was successfully cultured in vitro efficiently, which was with high growth capacity and short passage-period. After freezing and resuscitation, the fibroblasts could also well attach and proliferate, and could be provided as the appropriate donor cells for related nuclear transfer work. The tissue-pieced method was simple and effective access to the pig ear fibroblasts, the cells could be obtained in vitro for at least 4 generations could be used for donor cells for nuclear transfer embryos.