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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (31): 137-140.

所属专题： 生物技术； 烟草种植与生产； 农业生态

• 林学 园艺 园林 • 上一篇    下一篇

拟南芥青枯病抗性基因RRS1的烟草同源性检测

刘磊 阮龙 钱益亮 吴志超 韩漾 孙学永 王荣富   

  • 收稿日期:2012-04-23 修回日期:2012-05-03 出版日期:2012-11-05 发布日期:2012-11-05
  • 基金资助:

    烟草优质抗青枯病聚合育种及其抗性基因分析;烟草青枯病抗性的分子生物学研究

Detection on RRS1 gene of Bacterial wilt resistance from Arabidopsis in Tobacco for Homolog

  • Received:2012-04-23 Revised:2012-05-03 Online:2012-11-05 Published:2012-11-05

PDF (PC)

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摘要：

旨在进行烟草种质抗青枯病基因RRS1的筛选研究，于2008年、2009年及2011年选取国内外80个烟草品种，开展大田青枯病病害指数调查及RRS1基因9对特异引物的筛选工作，通过9对引物及80个烟草种质的筛选，目前已经鉴定出引物6F-6R (5′-ATGAGAAAGAGGCTCGTCAA-3′和5′-ACCACAACCCTCAAGCAGTT-3′)能够有效地筛选种质，共筛选出3个烟草种质(G28、K346、LMAFC34)含有RRS1特异性扩增序列；针对引物(6R-6F)扩增的3个烟草种质材料的特异性序列进行测序，开展与RRS1基因的cDNA序列的同源性比对，为烟草种质资源的RRS1基因同源性评价奠定工作基础。

关键词: 血液流变学常值, 血液流变学常值

Abstract:

It is aimed to study and help for selecting the germplasm of the resistance gene of the tobacco ralstonia solanacearum in RRS1 gene. The investigation and selection was carried out in 2008, 2009 and 2011. Eighty tobacco germplasm resources were chosen in the world. The investigation to the index of ralstonia solanacearum in tobacco was carried out in the field. The nine pairs of specific primers were selected to screen for the RRS1 gene. Through the selection, the primer 6F-6R (5′-ATGAGAAAGAGGCTCGTCAA-3′ and 5′-ACCACAACCCTCAAGCAGTT-3′) was sure to pick out the available germplasm. Three germplasm of tobacco was selected for containing the specific amplified sequence of RRS1 for G28, K346 and LMAFC34. The specific sequences amplified by 6R-6F, were sequenced in 3 tobacco germplasm resources, and the homology of RRS1 cDNA was compared, these works established the base for assessing the homology of RRS1 gene.