欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (34): 184-189.

所属专题： 生物技术； 园艺

• 林学 园艺 园林 • 上一篇    下一篇

辣椒Arf GAP基因的克隆与表达分析

冯冬林 赖燕 何水林   

  • 收稿日期:2012-08-02 修回日期:2012-10-18 出版日期:2012-12-05 发布日期:2012-12-05

Cloning, Characterization and Expression of an ARF-GAP Gene from Pepper (Capsicum annuum L.)

  • Received:2012-08-02 Revised:2012-10-18 Online:2012-12-05 Published:2012-12-05

PDF (PC)

892

摘要：

ADP核糖基化因子(Arf) GTP酶激活蛋白(GAPs)能够促使束缚于Arfs的GTP水解为GDP，通过转化具有活性的GTP束缚型为非活性的GDP束缚型从而达到调节Arfs的作用。本研究从辣椒幼苗叶片cDNA文库中筛选获得一个阳性克隆。核苷酸序列分析表明该基因编码一个含有408个氨基酸残基，分子量约为43.98 kD，含有一个保守的Arf GAP结构域，与葡萄、大豆中的AGD8-Like基因的同源性分别为73%和71%，与拟南芥AtAGD8同源性为65%，命名为CaAGD8。实时荧光定量PCR检测表明，与对照相比，CaAGD8基因的相对表达量在辣椒茎、叶、花及果实中表达量升高。

关键词: 多酚氧化酶, 多酚氧化酶

Abstract:

ADP ribosylation factor (Arf) GTPase-activating proteins (GAPs) promote the hydrolysis of GTP bound to Arfs to GDP, which plays a pivotal role in regulating Arfs by converting the active GTP-bound forms of these proteins into their inactive GDP-bound forms. The positive clone was screened from was screening and isolated from cDNA library of seedling leaves of pepper and was designated as CaAGD8. Sequence analysis showed that the 43.98 kD proteins encoded a polypeptide of 408 amino acid residues and contain an Arf GAP domain. The CaAGD8 with 73% and 71% similarity respectively to the AGD8-Like of grape and soybean and with 65% similarity to the AtAGD8. The real time PCR results showed that compared with control CaAGD8 relative expression was higher in stems, leaves, flowers and fruits of pepper.