不同培养液对鹅胚胎体外培养的影响

doi:10.11924/j.issn.1000-6850.2012-2688

中国农学通报 ›› 2013, Vol. 29 ›› Issue (8): 18-20.doi: 10.11924/j.issn.1000-6850.2012-2688

不同培养液对鹅胚胎体外培养的影响

李玉芳庄益芬张文昌

收稿日期:2012-08-02 修回日期:2012-12-03 出版日期:2013-03-15 发布日期:2013-03-15
基金资助:
国家科技支撑计划项目“动物快繁关键技术研究与示范”(2011BAD19B04)；福建省自然科学基金项目“以鹌鹑干细胞为载体制作转人血清白蛋白基因鹌鹑的研究”(2007J0042)

Effects of Different Mediums on Goose Embryos Culture in vitro

Received:2012-08-02 Revised:2012-12-03 Online:2013-03-15 Published:2013-03-15

摘要/Abstract

摘要： 为了探讨不同培养液对鹅胚胎体外培养的影响，本试验分别用鸡、鸭、鹅3种稀蛋白作培养液对90只鹅胚进行体外培养。结果表明：用鹅稀蛋白作培养液优于鸡稀蛋白和鸭稀蛋白。鹅稀蛋白组在第6、15天时鹅胚存活率显著高于鸭稀蛋白组(P<0.05)，鹅胚存活率在第6、15天分别为33.33%、20.00%；在第31天时鹅稀蛋白组鹅胚存活率为3.33%，其他两组为0。综上所述，鹅胚体外培养效果因培养液的不同而不同，在鸡、鸭和鹅稀蛋白3种培养液中，以选用鹅稀蛋白为宜。

关键词: 城乡统筹, 城乡统筹

Abstract: The studies were conducted to investigate the effect of different medium on goose embryo in vitro, 90 goose embryos from natural eggs were cultured in 3 different mediums. The results showed that: the dilute protein of goose eggs (GP) used as culture medium was better than that of chicken (CP) and duck (DP). Survival rate of goose embryo in GP was significantly higher than that in DP at 6th and 15th days (P<0.05), which were 33.33% and 20% respectively. Survival rate of goose embryo in GP was 3.33% at 31 th days, while the survival rate was 0 in other 2 mediums. All the analysis showed that the effect of different medium on goose embryo in vitro because of different culture medium, which in 3 different mediums, GP was suitable.

李玉芳庄益芬张文昌. 不同培养液对鹅胚胎体外培养的影响[J]. 中国农学通报, 2013, 29(8): 18-20.

参考文献

[1] 桑滋润.动物繁殖生物技术 [M].北京:中国农业出版社,2002: 396-400.
[2] Harvey A J. Expression of exogenous protein in the egg white of transgenic chickens[J].Nat. Biotechnol,2002(20):396-399.
[3] Harvey A J. Consistent production of transgenic chickens using replication deficient retroviral vectors and highthrouhput screening procedures[J].Poult. Sci,2002(81):202-212.
[4] Ivarie R. Avian transgenesis: Progress towards the promise[J]. Trends Biotechnol,2003(21):14-19.
[5] Dunn B E. Technique for shell-less culture of the 72-hour avian embryo. Poult[J].Sci,1974(53):409-412.
[6] Rowlett K, Simkiss K. Explanted embryo culture in vitro and vivo techniques for domestic fowl[J].British poul. Sci,1987(28):91-101.
[7] Ono T, Wakasugi N. Mineral content of quail embryos cultured in mineral-rich and mineral-free conditions[J].Poult. Sci,1984(63): 159-166.
[8] Perry M M. A complete culture system for the chick embryo[J]. Nature,1988(331):70-72.
[9] Natio M, Nirasawn K, Oishi T. Development in culture of the chick embryo from fertilized ovum to hatching[J].Journal of Experimential Zoology,1990,254(3):322-326.
[10] 李赞东,杜国清,周顺王,等.在代用蛋壳中孵化鸡胚[J].畜牧兽医学报,1994,25(6):481-484.
[11] 张文昌.鸡胚胎换蛋壳培养[J].中国家禽,1991(6):31-32.
[12] 李碧春,陈国宏,吴圣龙,等.不同时期鸡胚胎体外培养的比较研究[J].中国家禽,2004,26(14):14-16.
[13] 李玉芳,庄益芬,徐芹伟,等.鹌鹑胚胎的换壳培养[J].福建畜牧兽医, 2006,28(2):4-5.
[14] 张文昌,徐国正,庄益芬,等.鸭胚胎代用蛋壳培养的研究[J].中国农学通报,2007,23(11):8-11.
[15] 刘凤军,庄益芬,张文昌.鹅胚胎体外培养的研究[J].黑龙江畜牧兽医,2004(1):15-17.
[16] 邱祥聘.家禽学[M].成都:四川科学技术出版社,1993:96.

不同培养液对鹅胚胎体外培养的影响

Effects of Different Mediums on Goose Embryos Culture in vitro

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