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中国农学通报 ›› 2013, Vol. 29 ›› Issue (33): 129-134.doi: 10.11924/j.issn.1000-6850.2012-3833

• 农学 农业基础科学 • 上一篇    下一篇

基于SSR标记的赤峰地区甜荞种质的遗传多样性研究

杨学文 丁素荣 胡陶 刘迎春 张晓荣 生国利   

  • 收稿日期:2012-11-26 修回日期:2012-12-23 出版日期:2013-11-25 发布日期:2013-11-25
  • 基金资助:
    内蒙古自治区科技计划项目

SSR Marker-based Genetic Diversity of Common Buckwheat Accessions from Chifeng

  • Received:2012-11-26 Revised:2012-12-23 Online:2013-11-25 Published:2013-11-25

摘要: 内蒙古赤峰地区是我国甜荞的主产区之一。为了研究赤峰地区甜荞种质的遗传多样性、合理利用育种材料,采用SSR分子标记对大部分来自赤峰地区的73份甜荞种质的遗传多样性进行了分析。15对引物共扩增出77个条带,其中多态性条带有50条,占总数的64. 9%。大部分材料之间的遗传相似系数在0.78~0.96之间。在相似系数为0.81时,73份材料可分成4组,赤峰地区的58份材料在每一组中均有分布,表明赤峰地区的甜荞具有丰富的遗传多样性。

关键词: 综合利用, 综合利用

Abstract: Chifeng in Inner Mongolia is one of the major producing areas of common buckwheat in China. In order to study the biodiversity of common buckwheat accessions from Chifeng and make good use of the materials for breeding, we analyzed the genetic diversity and relationship among seventy-three common buckwheat accessions using fifteen pairs of SSR primers. Seventy-seven bands were amplified, fifty of which were polymorphic, which accounted for 64. 9%. Genetic coefficient of most accessions ranged from 0.78 to 0.96 by software NTSYSpc2.1 based on SSR results. The seventy-three common buckwheat accessions could be divided into four groups on coefficient threshold of 0.81. The fifty-eight accessions from Chifeng were found in all groups, indicating the high biodiversity of the accessions.