欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2013, Vol. 29 ›› Issue (28): 150-155.doi: 10.11924/j.issn.1000-6850.2013-0350

• 林学 园艺 园林 • 上一篇    下一篇

无籽罗汉果组培无菌系的构建

潘丽梅 莫长明 马小军 冯世鑫 唐其 白隆华 韦荣昌 邢爱佳   

  • 收稿日期:2013-02-01 修回日期:2013-03-29 出版日期:2013-10-05 发布日期:2013-10-05
  • 基金资助:
    罗汉果质量改进和综合开发利用;罗汉果、佛手、青蒿种子（苗）质量标准研究;无籽罗汉果产量和品种促控技术研究;无籽罗汉果遗传转化体系的构建

The Construction of the Aseptic Strain of the Tissue Culture of Seedless siraitia grosvenorii

  • Received:2013-02-01 Revised:2013-03-29 Online:2013-10-05 Published:2013-10-05

PDF (PC)

758

摘要： 【研究目的】研究无籽罗汉果组培快繁技术要点，为无籽罗汉果再生体系建立和工厂化生产提供技术支持。【方法】以幼嫩茎段为试验材料，探讨不同灭菌时间，不同激素组合以及浓度对启动培养、增殖培养、生根培养等的影响。【结果】无籽罗汉果带芽茎段最佳消毒方法为1% HgCl2溶液消毒8 min。最适宜的无籽罗汉果茎段启动培养基为MS+1.0 mg/L 6-BA+1.5 mg/L KT，无籽罗汉果继代增殖培养基为MS+1.0 mg/L 6-BA+0.5 mg/L IBA，增殖系数可达6.8；较适合生根的培养基为1/2MS+1.0 mg/L IBA+0.5 mg/LNAA，新稍平均生根数达7.4条，生根率可达95%。【结论】可为无籽罗汉果大规模组织培养和快速繁殖提供理论基础，并为组织培养高效体系的建立及外源基因导入提供参考。

关键词: 综合利用, 综合利用

Abstract: 【Objective】This research explored the tissue culture and rapid propagation of seedless Siraitia grosvenorii in order to provide technical supports on seedless Siraitia grosvenorii regenerations system establishment and factory production .【Method】The young stem of seedless Siraitia grosvenorii was used as explants to investigate the effects of different sterilization time, different hormone combinations and concentrations on the initiating, proliferation, as well as rooting cluster buds were studied.【Result】The results showed that the best sterilization method for the stem segments was agitating in 0.1% HgCl2 for 8 min. The optimum medium for initiation culture of stem segments was MS+ 1.0 mg/L 6-BA+1.5 mg/L KT , The suitable multiplication medium was MS+1.0 mg/L 6-BA+0.5 mg/L IBA, with the bud proliferation coefficient of 6.8; The suitable rooting medium was 1/2MS+1.0 mg/L IBA+0.5 mg/L NAA, in which the average root number was 7.4, the rooting rate of muhiplied buds was up to 95%.【Conclusion】It could provide theoretical basis for the large-scale production of the tissue culture and rapid propagation of seedless Siraitia grosvenorii, and the experiment provided references for the establishment of efficient Tissue culture regeneration system and the exogenous gene transduction.