影响烟草香气物质合成代谢途径关键酶基因的研究进展

doi:10.11924/j.issn.1000-6850.2013-1481

摘要/Abstract

摘要： 文章详细叙述了与植物香气物质合成有关的3类主要合成代谢途径，并分别归纳苯丙烷代谢途径、异戊二烯代谢途径和生物碱合成途径中催化主要中间产物形成的关键酶基因功能与表达模式，重点阐述关键酶基因在烟草中的最新研究进展，提出目前除了在基因水平上进行表达水平调控、转基因作物改良之外，烟草中亟待进行更为深入的蛋白水平研究和代谢网络互作分析。通过上述分析，对下一步利用代谢工程改良烟草香气品质及其在农业生产领域的应用前景进行了展望。

关键词: RCA, RCA

Abstract: This article specified three main kinds of regulating pathway related with plant aroma compounds synthesis, concluded the function and expression types of key enzyme genes in catalyzing major intermediate products which belong to phenyl propane metabolic pathways, isoprene metabolic pathways and alkaloid synthesis pathway respectively, and highlighted the latest developments of these key enzyme genes in tobacco, Accordingly this article raised that more in-depth research in protein as well as the metabolic network interaction analysis is urgently needed, in addition to the expression regulation and genetically modified crop improvement at the genetic level. From above analysis, next improvement of tobacco fragrance quality through metabolic engineering and its application prospect in agricultural production areas were prospected.

吕婧刘贯山晁江涛孙玉合. 影响烟草香气物质合成代谢途径关键酶基因的研究进展[J]. 中国农学通报, 2014, 30(4): 49-57.

参考文献

[1] Taguchi G, Sharan M, Gonda K, et al. Effect of methyl jasmonate and elicitor on PAL gene expression in tobacco cultured cells[J]. Journal of Plant Biochemistry and Biotechnology,1998,7:79-84.
[2] Edwards K, Cramer C L, Bolwell G P, et al. Rapid transient induction of phenylalanine ammonia-lyase mRNA in elicitor-treated bean cells[J]. Proceedings of the National Academy of Sciences, 1985,82(20):6731-5.
[3] Nagai N, Kitauchi F, Shimosaka M, et al. Cloning and sequencing of a full-length cDNA coding for phenylalanine ammonia-lyase from tobacco cell culture[J]. Plant physiology,1994,104(3):1091.
[4] Brederode F T, Linthorst H J M, Bol J F. Differential induction of acquired resistance and PR gene expression in tobacco by virus infection, ethephon treatment, UV light and wounding[J]. Plant molecular biology, 1991,17(6): 117-125.
[5] Reichert A, He X, Dixon R. Phenylalanine ammonia-lyase (PAL) from tobacco (Nicotiana tabacum): characterization of the four tobacco PAL genes and active heterotetrameric enzymes1[J]. Biochem J,2009,424:233-242.
[6] Schilmiller A L, Stout J, Weng J K, et al. Mutations in the cinnamate 4-hydroxylase gene impact metabolism, growth and development in Arabidopsis[J]. The Plant Journal,2009, 60(5): 771-82.
[7] Szatmari A, Ott P G, Varga G J, et al. Characterisation of basal resistance (BR) by expression patterns of newly isolated representative genes in tobacco [J]. Plant cell reports,2006,25(7): 728-740.
[8] Hu W J, Kawaoka A, Tsai C J, et al. Compartmentalized expression of two structurally and functionally distinct 4-coumarate: CoA ligase genes in aspen (Populus tremuloides) [J]. Proceedings of the National Academy of Sciences,1998,95(9): 5407-5412.
[9] Stuible H P, Kombrink E. Identification of the substrate specificity-conferring amino acid residues of 4-coumarate: coenzyme A ligase allows the rational design of mutant enzymes with new catalytic properties [J]. Journal of Biological Chemistry, 2001,276(29):26893-26897.
[10] Lee D, Douglas C J. Two Divergent Members of a Tobacco 4-Coumarate: Coenzyme A Ligase (4CL) Gene Family (cDNA Structure, Gene Inheritance and Expression, and Properties of Recombinant Proteins) [J]. Plant physiology,1996,112(1):193-205.
[11] Lee D, Ellard M, Wanner L A, et al. The Arabidopsis thaliana 4-coumarate: CoA ligase (4CL) gene: stress and developmentally regulated expression and nucleotide sequence of its cDNA [J]. Plant molecular biology,1995,28(5):871-84.
[12] Durbin M L, Mccaig B, Clegg M T. Molecular evolution of the chalcone synthase multigene family in the morning glory genome [J]. Plant molecular biology,2000,42(1):79-92.
[13] Leyva A, Jarillo J A, Salinas J, et al. Low temperature induces the accumulation of phenylalanine ammonia-lyase and chalcone synthase mRNAs of Arabidopsis thaliana in a light-dependent manner [J]. Plant physiology,1995,108(1):39-46.
[14] Nishihara M, Nakatsuka T, Yamamura S. Flavonoid components and flower color change in transgenic tobacco plants by suppression of chalcone isomerase gene [J]. FEBS letters,2005,579(27):6074-8.
[15] Muir S R, Collins G J, Robinson S, et al. Overexpression of petunia chalcone isomerase in tomato results in fruit containing increased levels of flavonols[J]. Nature biotechnology,2001,19(5):470-474.
[16] 王源,黄丛林,张秀海,等.转录因子基因 ZmDREB3转化玉米的研究[J].作物杂志,2012,3:15.
[17] Eisenreich W, Sagner S, Zenk M H, et al. Monoterpenoid essential oils are not of mevalonoid origin[J]. Tetrahedron letters,1997,38 (22):3889-3892.
[18] Eisenreich W, Menhard B, Hylands P J, et al. Studies on the biosynthesis of taxol: the taxane carbon skeleton is not of mevalonoid origin [J]. Proceedings of the National Academy of Sciences,1996,93(13):6431-6436.
[19] Adam K P, Zapp J. Biosynthesis of the isoprene units of chamomile sesquiterpenes[J]. Phytochemistry,1998,48(6):953-989.
[20] Zeidler J, Lichtenthaler F, May H. Is isoprene emitted by plants synthesized via the novel isopentenyl pyrophosphate pathway? [J]. Zeitschrift für Naturforschung C A journal of biosciences,1997,52 (1-2):15-23.
[21] Eisenreich W, Bacher A, Arigoni D, et al. Biosynthesis of isoprenoids via the non-mevalonate pathway[J]. Cellular and Molecular Life Sciences CMLS, 2004,61(12):1401-1426.
[22] Pulido P, Perello C, Rodriguez-concepcion M. New Insights into Plant Isoprenoid Metabolism [J]. Molecular plant,2012,5(5): 964-967.
[23] Flores-p Rez ú, P Rez-gil J, Closa M, et al. Pleiotropic Regulatory Locus 1 (PRL1) integrates the regulation of sugar responses with isoprenoid metabolism in Arabidopsis[J]. Molecular plant,2010,3(1): 101-112.
[24] Choi D, Ward B L, Bostock R M. Differential induction and suppression of potato 3-hydroxy-3-methylglutaryl coenzyme A reductase genes in response to Phytophthora infestans and to its elicitor arachidonic acid[J]. The Plant Cell Online,1992,4(10): 1333-1344.
[25] Nelson A J, Doerner P W, Zhu Q, et al. Isolation of a monocot 3-hydroxy-3-methylglutaryl coenzyme A reductase gene that is elicitor-inducible [J]. Plant molecular biology,1994,25(3):401-412.
[26] Enjuto M, Lumbreras V, Mar N C, et al. Expression of the Arabidopsis HMG2 gene, encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase, is restricted to meristematic and floral tissues [J]. The Plant Cell Online,1995,7(5):517-527.
[27] Lluch M A, Masferrer A, Arr M, et al. Molecular cloning and expression analysis of the mevalonate kinase gene from Arabidopsis thaliana[J]. Plant molecular biology,2000,42(2): 365-376.
[28] Schulte A E, Van der Heijden R, Verpoorte R. Purification and Characterization of Mevalonate Kinase from Suspension-Cultured Cells of Catharanthus roseus (L.) G. Don [J]. Archives of biochemistry and biophysics,2000,378(2):287-298.
[29] Gupta S D, Fang J, Brake S S, et al. Biosynthesis of Sterols and Wax Esters by Euglen of Acid Mine Drainage Biofilms: Implications for Eukaryotic Evolution and the Early Earth[J]. Chemical Geology,2012.
[30] Est Vez J M, Cantero A, Reindl A, et al. 1-Deoxy-D-xylulose-5-phosphate synthase, a limiting enzyme for plastidic isoprenoid biosynthesis in plants[J]. Journal of Biological Chemistry,2001,276(25):22901-22909.
[31] Cordoba E, Porta H, Arroyo A, et al. Functional characterization of the three genes encoding 1-deoxy-D-xylulose 5-phosphate synthase in maize [J]. Journal of experimental botany,2011,62(6):2023-2038.
[32] Walter M H, Hans J, Strack D. Two distantly related genes encoding 1-deoxy-d-xylulose 5-phosphate synthases: differential regulation in shoots and apocarotenoid-accumulating mycorrhizal roots[J]. The Plant Journal,2002,31(3):243-254.
[33] Phillips M A, Walter M H, Ralph S G, et al. Functional identification and differential expression of 1-deoxy-D-xylulose 5-phosphate synthase in induced terpenoid resin formation of Norway spruce (Picea abies) [J]. Plant molecular biology,2007,65 (3):243-257.
[34] Carretero-paulet L, Ahumada I, Cunillera N, et al. Expression and Molecular Analysis of the Arabidopsis DXR Gene Encoding 1-Deoxy-d-Xylulose 5-Phosphate Reductoisomerase, the First Committed Enzyme of the 2-C-Methyl-d-Erythritol 4-Phosphate Pathway[J]. Plant physiology,2002,129(4):1581-1591.
[35] Hans J, Hause B, Strack D, et al. Cloning, characterization, and immunolocalization of a mycorrhiza-inducible 1-deoxy-D-xylulose 5-phosphate reductoisomerase in arbuscule-containing cells of maize [J]. Plant physiology,2004,134(2):614-624.
[36] Rohdich F, Wungsintaweekul J, Eisenreich W, et al. Biosynthesis of terpenoids: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase of Arabidopsis thaliana[J]. Proceedings of the National Academy of Sciences,2000,97(12):6451-6456.
[37] Rohdich F, Wungsintaweekul J, L Ttgen H, et al. Biosynthesis of terpenoids: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase from tomato[J]. Proceedings of the National Academy of Sciences, 2000,97(15):8251-8256.
[38] Roberts S C. Production and engineering of terpenoids in plant cell culture[J]. Nature chemical biology,2007,3(7):387-395.
[39] Baldwin I T. Mechanism of damage-induced alkaloid production in wild tobacco[J]. Journal of Chemical Ecology,1989,15(5): 1661-1680.
[40] Sato F, Hashimoto T, Hachiya A, et al. Metabolic engineering of plant alkaloid biosynthesis[J]. Proceedings of the National Academy of Sciences,2001,98(1):367-372.
[41] Winz R A, Baldwin I T. Molecular Interactions between the Specialist Herbivore Manduca sexta (Lepidoptera, Sphingidae) and Its Natural Host Nicotiana attenuata. IV. Insect-Induced Ethylene Reduces Jasmonate-Induced Nicotine Accumulation by Regulating Putrescine N-Methyltransferase Transcripts[J]. Plant physiology, 2001,125(4):2189-2202.
[42] Mizusaki S, Tanabe Y, Noguchi M, et al. Changes in the activities of ornithine decarboxylase, putrescine N-methyltransferase and N-methylputrescine oxidase in tobacco roots in relation to nicotine biosynthesis [J]. Plant and Cell Physiology,1973,14(1):103-110.
[43] Sato F, Hashimoto T, Hachiya A, et al. Metabolic engineering of plant alkaloid biosynthesis[J]. Proceedings of the National Academy of Sciences,2001,98(1):367-372.
[44] Takeshita N, Fujiwara H, Mimura H, et al. Molecular cloning and characterization of S-adenosyl-L-methionine: scoulerine-9-O-methyltransferase from cultured cells of Coptis japonica[J]. Plant and Cell Physiology,1995,36(1):29-36.
[45] Dubouzet J G, Morishige T, Fujii N, et al. Transient RNA silencing of scoulerine 9-O-methyltransferase expression by double stranded RNA in Coptis japonica protoplasts[J]. Bioscience, biotechnology, and biochemistry,2005,69(1):63-70.
[46] Conkling M A. Modifying nicotine and nitrosamine levels in tobacco[M]. Google Patents,2005.
[47] Ohara K, Ujihara T, Endo T, et al. Limonene production in tobacco with Perilla limonene synthase cDNA[J]. Journal of experimental botany,2003,54(393):2635-2642.