欢迎访问《中国农学通报》,

中国农学通报 ›› 2014, Vol. 30 ›› Issue (18): 259-263.doi: 10.11924/j.issn.1000-6850.2013-2611

所属专题: 生物技术

• 生物技术科学 • 上一篇    下一篇

副干酪乳杆菌prcA基因克隆及生物信息学分析

王洋 葛菁萍 由田 平文祥   

  • 收稿日期:2013-10-08 修回日期:2013-12-17 出版日期:2014-06-25 发布日期:2014-06-25
  • 基金资助:
    国家自然科学基金“副干酪乳杆菌HD1.7细菌素的产生与菌群密度之间的生态学效应关系的研究”(31070446);国家自然科学基金“拮抗菌株对副干酪乳杆菌产细菌素的影响及关键蛋白质的筛选”国际合作项目(31210103026);国家自然科学基金“表达IBDV主要保护性抗原的重要杆状病毒构建及其作为疫苗的免疫效果研究”(31270143);国家自然科学基金“菌群演替与温水沤麻系统关键酶产生菌代谢组学特征的耦合机制”(31270534);黑龙江大学高层次人才(创新团队)支持计划“功能微生物选育及其产物应用”(Hdtd2010-17);黑龙江省科技厅“农业微生物发酵技术”科技创新团队,黑龙江省高等学校科技创新团队(农业微生物发酵技术)。

Cloning and Bioinformatics Analysis of prcA in Lactobacillus paracasei

ping jingge   

  • Received:2013-10-08 Revised:2013-12-17 Online:2014-06-25 Published:2014-06-25

摘要: 旨在了解副干酪乳杆菌prcA基因的结构和功能,探明prcA基因与副干酪乳杆菌拟群体效应相关基因—自诱导肽编码基因之间的联系。通过PCR方法对副干酪乳杆菌中prcA进行扩增,并通过生物信息学方法对该基因的核苷酸组成、蛋白质构象等方面进行预测和分析。克隆得到的prcA基因全长为416 bp,包括一个138 bp的开放阅读框,编码45个氨基酸,蛋白分子量为5326.83 Da,等电点为4.85,是一种稳定蛋白,不含跨膜区,蛋白质的二级结构以α-螺旋、不规则卷曲和延伸主链组成,亚细胞定位预测显示该蛋白主要位于细胞外;prcA基因为副干酪乳杆菌拟群体效应相关基因—自诱导肽编码基因,负责细菌素生成的调控。

关键词: 土地利用效益, 土地利用效益, 城市边缘区, 评价, 大兴区

Abstract: The aims were to investigate the structure and function of prcA gene in Lactobacillus paracasei, and find out the relationship between prcA gene and autoinducing peptide gene of putative quorum sensing related genes in Lactobacillus paracasei. prcA gene was cloned by polymerase chain reaction (PCR) and predicted by bioinformatic methods. The prcA was 416 bp, containing a 138 bp open reading frame (ORF), which encoded 45 amino acids, while the estimated molecular weight and isoelectric point of the putative protein were 5326.83 Da and 4.85, it was a stability protein without membrane- spanning domain, alpha helix, random coil and extended strand were major structural elements of polypeptide chain, subcellular localization result indicated that it was located outside the cell, prcA is the putative quorum sensing related gene in Lactobacillus paracasei encoding autoinducing peptide, which regulates bacteriocin production.