从广东省各蔬菜基地采集101个表层土壤,测定土壤Olsen-P、Bray-P和CaCl2-P,以确定不同质地土壤的磷淋失临界值。结果表明,Olsen-P与CaCl2-P呈显著的线性关系,随着土壤Olsen-P的增加,CaCl2-P也呈现增加的趋势,存在1个明显的突变点,即土壤磷淋失临界值,壤质粘土的临界值为79.74 mg/kg,粘壤土的临界值为51.08 mg/kg,砂质粘壤土的淋失临界值为43.99 mg/kg,砂质壤土的淋失临界值为8.63 mg/kg,淋失临界值从高到低的顺序依次是:壤质粘土>粘壤土>砂质粘壤土>砂质壤土,与不同质地粘粒含量的表现规律一致。在砂质粘壤土上Bray-P与CaCl2-P呈显著的线性关系,但不存在明显的突变点,在壤质粘土、粘壤土和砂质壤土上,Bray-P与CaCl2-P未呈现显著的线性关系,不存在明显的突变点。因此,在本试验条件下,Olsen-P可以作为不同质地土壤磷淋失临界值指标。
[1] R. James Cook. Biological Control of Plant Pathogens: Theory to Application[J].Phytopathology,1985,75(1):25-29.
[2] Kloepper J W, Schroth M N. Plant Growth Promoting Rhizobacteria in Radish[A].Proceedings of the 4th International Conference of Plant Pathogenic Bacteria[C].GilbertClarey, Tours, France,1978: 879-882.
[3] 胡江春,薛德林,马成新.植物根际促生菌(PGPR)的研究与应用前景[J].应用生态学报,2004,15(10):1963-1966.
[4] Dworkin M, Foster J. Experiments with some microorganisms which utilize ethane and hydrogen[J].Bacteriol,1958(75):592-601.
[5] 陆雅海,张福锁.根际微生物研究进展[J].土壤,2006,38(2):113-121.
[6] Ownley B H, Benson D M. In: Trigiano R N, Windham M T, Windham A S, eds. Plant Pathology, Concepts and Laboratory Exercises (2nd ed)[M].Boca Raton, Florida, USA:CRC Press Inc., 2008:217-233.
[7] 李世东,缪作清,高卫东.我国农林园艺作物土传病害发生和防治现状及对策分析[J].中国生物防治学报,2011,27(4):433-440.
[8] 于淑池.拮抗细菌在生防领域的应用概述[J].承德职业学院学报, 2007,2:151-153.
[9] 陈志谊,张荣胜.植物病害生防芽孢杆菌研究进展[J].江苏农业学报,2012.28(5):999-1006.
[10] 郝晓娟.作物枯萎病生防细菌的研究[D].杭州:浙江大学,2006:9.
[11] 胡桂萍,郑雪芳,尤,民等.植物内生菌的研究进展[J].福建农业学报, 2010,25(2):226-234.
[12] 石晶盈,陈维信,刘爱媛.植物内生菌及其防治植物病害的研究进展[J].生态学报,2006,26(7):2395-2401.
[13] 李长松.拮抗性细菌生物防治植物土传病害的研究进展[J].生物防治通报,1992,8(4):168-172.
[14] 郭荣君,刘杏忠,杨怀文.应用根际细菌防治植物寄生线虫的研究[J].中国生物防治,1996,12(3):134-137.
[15] 路宗岩.杉木炭疽病拮抗细菌的筛选及其菌剂研制[D].湖南:中南林业科技大学,2013:2-3.
[16] 朱玥妍,刘娇,杜春梅.芽孢杆菌生物防治植物病害研究进展[J].安徽农业科学,2012,40(34):16635-16658.
[17] Hossain, Mohammad B. Inhibition of conidial growth of Venturia inaequalis by the extracellular protein fraction from the antagonistic bacterium Pseudomonas fluorescens Bk3[J].Biological Control,2009,48(2):133-139.
[18] 陈宝如,詹儒林,何红,等.红树内生细菌AiL3菌株鉴定及其胞外抗菌活性物质特性[J].农业生物技术学报,2010,18(4):801-806.
[19] 徐刘平,尹燕妮,李师默,等.拮抗细菌对土传病原菌的作用机理[J].中国生物防治,2006,22(1):10-14.
[20] 潘朝勃,莫贱友,李其利,等.拮抗细菌对芒果炭疽病生物防治研究进展[J].南方农业科学学报,2013,44(4):602-606.
[21] 王光华,Jos M.Raaijmakers.生防细菌产生的拮抗物质及其在生物防治中的作用[J].应用生态学报,2004,15(6):1100-1104.
[22] Kanchalee Jetiyanona, Joseph W Kloepperb. Mixtures of plant growth-promoting rhizobacteria for induction of systemic resistance against multiple plant diseases[J].Biological Control,2002,24(3): 285-291.
[23] Baker R. Diversity in biological control[J].Crop Protection,1991 (10):85-94.
[24] Blakeman J P. Microbial competition for nutrients and germination of fungal spores[J].Ann App Biol,1978,89:151-155.
[25] 杨秀荣,刘水芳,孙淑琴.生防细菌防治土传病害的研究进展[J].天津农业科学,2008,14(4):38-42.
[26] Ryals Neuenschwander, Willits, et al. Systemic acquired resistance [J].Plant Cell,1996,8:1887-1899.
[27] Martin, Ian, Baldwin. Fitness costs of induced resistance: emerging experimental support for a slippery concept[J].Trends in Plant Science,2002(2):31.
[28] 李钠钾.苯并噻二唑(BTH)处理烟苗对普通花叶病诱导抗性研究[D].河南:河南农业大学,2011:1-7.
[29] Benizri E, Baudoin E, Guckert A. Root Colonization by Inoculated Plant Growth- Promoting Rhizobacteria[J].Biocontrol Science and Technology,2001,11(5):557-579.
[30] Bahme, Jb, Schroth, Mn. Spatial-Temporal Colonization Patterns of A Rhizobacterium on Underground Organs of Potato[J]. Phytopathology,1987,77(7):1093-1100.
[31] Yaryura PM, León M, Correa O S, et al. Assessment of the role of chemotaxis and biofilm formation as requirements for colonization of roots and seeds of soybean plants by Bacillus amyloliquefaciens BNM339[J].Current Microbiology,2008,56(6):625-632.
[32] Hamon Morgan, Mbemba Elisabeth, Charnaux Nathalie, et al. A syndecan- 4CXCR4 complex expressed on human primary lymphocytes and macrophages and HeLa cell line binds the CXC chemokine stromal cell-derived factor-1 (SDF-1)[J].Glycobiology, 2004,14(4):311-323.
[33] 汪军,潘江禹,毛超,等.土壤物理因素和栽培方式对淡紫拟青霉E7在香蕉根际定殖和促生作用的影响[J].果树学报,2013,30(2):274- 280.
[34] 严婉荣,赵廷昌,肖彤斌,等.生防细菌在植物病害防治中的应用[J].基因组学与应用生物学,2013,32(4):533-539.
[35] Sanchez- Azofeifa, Yumi Oki, G. Wilson Fernandes, et al. Relationships between endophyte diversity and leaf optical properties[J].Trees-Structure and Function,2012,26(2):291-299.
[36] 张庭静.地黄内生菌的分离鉴定和产梓醇菌株的筛选及其发酵研究[D].河南:河南农业大学,2011:1-5.
[37] 胡炳福.两种抗生细菌防治林木土传病害研究初报[J],生物防治通报,1988,4(4):172-175.
[38] 张运强,谢艺贤,张辉强.橡胶树红根病病原菌的鉴定(II)[J].热带作物学报,2000,20(1):20-24.
[39] 伍慧雄,王胜坤,孙思.桉树青枯病的发生与防治[J].广东林业科技, 2006,22(3):53-55.
[40] 王建美,才玉石,陶万强,等.不同真菌菌株对黄栌的致病性研究[J].中国森林病虫,2008,27(3):1-3.
[41] 向玉英.杨树上发生的两种病毒[J].林业科学研究,1990,3(6):553- 557.
[42] 陈京元,徐红梅,蔡三山.松苗猝倒病不同病原物的致病性差异及其生防细菌的筛选[J].东北林业大学学报,2006.34(2):5-7.
[43] 张兴锋,柳凤.红树内生细菌CШ-1菌株鉴定及其保外抗菌蛋白性质[J].微生物学通报,2010,37(2):222-227.
[44] 金海强,贾斌,李熙英.杨树腐烂病内生拮抗细菌鉴定及防治研究[J].中国森林病虫,2012,31(6):1-4.
[45] 路宗岩.杉木炭疽病拮抗菌HY32的筛选及其应用[J].生物技术通报,2013,3:181-185.
[46] 张艳东,曹梦琪,王俊,等.桑树根腐病真菌病原拮抗细菌JK-7的分离与鉴定[J].蚕业科学,2013.39(2):0220-0225.
[47] Valiente C, Diaz K, Gacitúa S, et al. Control of charcoal root rot in Pinus radiata nurseries with antagonistic bacteria[J].World Journal of Microbiology and Biotechnology,2008,24(4):557-568.
[48] Carlos Marcelo Ribeiro, et al. Isolation, selection and characterization of root- associated growth promoting bacteria in Brazil Pine[J].Microbiological Research,2012,167(2):69-78.
[49] Elo S L. Maunuksela M. Salkinoja-Salonen, et al. Humus bacteria of Norway spruce stands: plant growth promoting properties and birch, red fescue and alder colonizing capacity[J].FEMS Microbiology Ecology,2000,31(2):143-152.
[50] Ren Jia-Hong, Li Hao, Wang Yan-Fang, et al. Biocontrol potential of an endophytic Bacillus pumilus JK-SX001 against poplar canker [J].Biological Control.2013,67(3):421-430.
[51] Ran L X, Liu CY , Wu G J, et al. Suppression of bacterial wilt in Eucalyptus urophylla by fluorescent Pseudomonas spp. in China[J]. Biological Control,2005,32:111-120.
[52] 陈海英,廖富蘋,林健荣,等.拮抗细菌在植物病害防治中的应用及展望[J].安徽农业科学,2008,36(20):8690-8691.
[53] 许彦君,刘海龙,刘新晶,等.细菌对植物病害生物防治研究进展[J].大豆科技,2011,05:18-24.
[54] Roose- Amsaleg C L, Garnier- sillam E, Harry M. Extraction and purification ofmicrobial DNA f rom soil and sediment samples[J]. Applied Soil Ecology,2001,18:47-60.
[55] Grayst on S J, Prosser J I. Impact of cult ivation on characterization of species composition of soil bacterial communities[J]. FEMSMicrobiology Ecology,2001,35(1):37-48.
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