家蚕化学感受蛋白BmCSP8表达谱及多克隆抗体制备

doi:10.11924/j.issn.1000-6850.2013-3039

中国农学通报 ›› 2014, Vol. 30 ›› Issue (19): 298-303.doi: 10.11924/j.issn.1000-6850.2013-3039

所属专题：生物技术

家蚕化学感受蛋白BmCSP8表达谱及多克隆抗体制备

邓培渊李玉华袁丹丹王国霞李长看

收稿日期:2013-11-19 修回日期:2014-04-10 出版日期:2014-07-05 发布日期:2014-07-05
基金资助:
国家自然基金“家蚕嗅觉受体与信息素结合蛋白相互作用的功能研究”(30700070)；郑州师范学院校内课题“郑州地区主要蔬菜害虫气味结合蛋白基因功能研究”(2012082)。

Expression Profiling and Preparation of Polyclonal Antibody of Chemosensory Protein BmCSP8 in the Silkworm, Bombyx mori

Received:2013-11-19 Revised:2014-04-10 Online:2014-07-05 Published:2014-07-05

摘要/Abstract

摘要： 为阐明昆虫化学感受蛋白(Chemosensory Proteins，CSPs)基因在昆虫不同组织部位和发育时期表达特性。运用半定量RT-PCR的方法分析了家蚕BmCSP8基因的时空表达谱。结果显示：（1）BmCSP8基因在雌、雄蛹中均表达,但在卵期不表达，在幼虫阶段，低龄期高于高龄期的表达量；（2）BmCSP8基因广泛分布于成虫身体各组织，但雌雄间的表达存在差异性，在主要感受器官触角中的表达量较低；运用重组BmCSP8蛋白成功制备兔多克隆抗体，Western-blot分析发现抗血清与触角蛋白发生特异性结合，为深入研究其生理功能打下基础。以上结果表明：BmCSP8基因可能参与了家蚕觅食的生理过程，且具有其他尚未明确的非嗅觉功能。

关键词: 高光谱, 高光谱, 反演, Hyperion, 水质参数, 东洞庭湖

Abstract: In order to clarify the expression characteristics of chemosensory protein (CSP) gene in different tissue parts and developmental stages in insect. The spatio-temporal expression profile of BmCSP8 was investigated using semi-quantitative RT-PCR. The results showed that: (1)BmCSP8 was expressed in larva and pupa stage, but not in egg stage. In larva stage, the expression was more in younger stages than that in older stages; (2) BmCSP8 was expressed in various adult tissues including antenna, head (without antenna), wing, leg, thorax and abdomen. But there were differences existing between female and male, and the expression level was low in antenna which was the primary sense organs of insect; (3) Rabbit polyclonal antibody was prepared successfully by recombinant BmCSP8 protein, Western-blot anlysis showed that antiserum could combined specifically with the antenna protein, it also provided a foundation for further research on the BmCSP8 physiological functions. These results suggested that BmCSP8 may has unknown non-olfactory functions, and most likely involved in the physiological process of foraging.

邓培渊李玉华袁丹丹王国霞李长看. 家蚕化学感受蛋白BmCSP8表达谱及多克隆抗体制备[J]. 中国农学通报, 2014, 30(19): 298-303.

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家蚕化学感受蛋白BmCSP8表达谱及多克隆抗体制备

Expression Profiling and Preparation of Polyclonal Antibody of Chemosensory Protein BmCSP8 in the Silkworm, Bombyx mori

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