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中国农学通报

中国农学通报 ›› 2005, Vol. 21 ›› Issue (8): 318-318.

所属专题： 生物技术； 小麦

• 目次 • 上一篇    下一篇

小麦蓝矮病植原体的分子生物学检测研究

张 荣,孙广宇,张雅梅,张秦风,赵二龙   

  • 出版日期:2005-08-05 发布日期:2005-08-05

Molecular Detection of Phytoplasma associated with Wheat Blue Dwarf

Zhang Rong, Sun Guangyu, Zhang Yamei, Zhang Qinfeng, Zhao Erlong   

  • Online:2005-08-05 Published:2005-08-05

摘要： 小麦蓝矮病是中国北方小麦的重要病害。利用植原体特异引物对小麦蓝矮病带病植株总DNA进行巢式-PCR（Nested-PCR）扩增，获得1.2kb的特异性片段，从分子水平上证明小麦蓝矮病是一种植原体病害。对具有红矮和蓝矮症状的病株扩增均可扩增出目标片段，说明红矮和蓝矮症状都是由植原体引起。试验为在小麦蓝矮病分子诊断、田间介体带毒检测、寄主范围研究等提供一种快速、灵敏可靠的方法。

Abstract: Wheat Blue Dwarf is one of the main disease on winter wheat in Northwestern China. A 1.2kb fragment was amplified by phytoplasmas universal primers in total DNA sample extracted from diseased wheat with dark green leaves and red dwarf symptom by Nested-PCR. The result indicated molecularly that the pathogen of Wheat Blue Dwarf disease was phytoplasma. The different symptoms (dark green dwarf and red dwarf) were all caused by phytoplasmas. Nested-PCR could be as a rapid, exact technique for detecting the pathogens from wheat leaves, insect vectors and weed host.