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中国农学通报

中国农学通报 ›› 2015, Vol. 31 ›› Issue (22): 270-273.doi: 10.11924/j.issn.1000-6850.2014-1317

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响应面法优化乌苏里瓦韦热水提取绿原酸工艺

王丽红,姜晓婷,谭凤田   

  1. (佳木斯大学药学院,黑龙江佳木斯 154007）
  • 收稿日期:2014-05-07 修回日期:2015-07-03 接受日期:2015-05-22 出版日期:2015-08-20 发布日期:2015-08-20
  • 通讯作者: 王丽红
  • 基金资助:
    佳木斯大学研究生创新项目(LM2014_065)；佳木斯大学博士专项科研项目(BZ2015-06) 。

Optimization of Extraction Process of Chlorogenic Acid from Lepisorus ussuriensis (Regel et Maack) Ching in Hot Water by Response Surface Methodology

Wang Lihong, Jiang Xiaoting, Tan Fengtian   

  1. (College of Pharmacy, Jiamusi University, Jiamusi Heilongjiang 154007)
  • Received:2014-05-07 Revised:2015-07-03 Accepted:2015-05-22 Online:2015-08-20 Published:2015-08-20

摘要： 旨在对乌苏里瓦韦中绿原酸的工艺条件进行优化。采用热水提取乌苏里瓦韦中绿原酸，根据Box-Benhnken中心组合设计原理，设计了3因素3水平响应面分析试验，来确定乌苏里瓦韦中绿原酸的最佳工艺条件。结果表明乌苏里瓦韦绿原酸最佳提取工艺条件为：液料比25:1，提取时间4 min，提取温度96℃，在这种条件下绿原酸得率可达5.11%，与理论值相接近，表明响应面分析法优化乌苏里瓦韦中绿原酸热水提取工艺具有良好的效果。水提法具有能耗少、时间短和低成本等特点，为乌苏里瓦韦提取绿原酸的工业化生产提供了理论依据。

关键词: 农业科研, 农业科研, 课题经费, 财务管理, 预算编制, 资产管理

Abstract: The aim was to optimize the extraction process of chlorogenic acid from Lepisorus ussuriensis (Regel et Maack) Ching. The hot water was used to extract chlorogenic acid from Lepisorus ussuriensis (Regel et Maack) Ching. According to Box-Benhnken central composite design principles, three factors and three levels of response surface analysis tests were designed to determine the optimum extraction conditions of chlorogenic acid from Lepisorus ussuriensis (Regel et Maack) Ching. The results showed that the best extraction process of chlorogenic acid was as follows: liquid to material ratio was 25:1, extraction time was 4 min, and extraction temperature was 96℃. Under such condition, chlorogenic acid yield was up to 5.11% and close to the theoretical value, indicating that the hot water extraction process had good results with the response surface methodology to optimize the extraction process of chlorogenic acid from Lepisorus ussuriensis (Regel et Maack) Ching. The water extraction had characteristics such as low energy consumption, short time and low cost, which provided theoretical basis for industrialized production of chlorogenic acid from Lepisorus ussuriensis (Regel et Maack) Ching.