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中国农学通报 ›› 2014, Vol. 30 ›› Issue (31): 308-315.doi: 10.11924/j.issn.1000-6850.2014-1637

所属专题: 生物技术 植物保护 园艺

• 植物保护 农药 • 上一篇    下一篇

叶面肥喷施宝对芥蓝菜农药残留及抗氧化酶活性的效应

汤鸣强,黄伙水,姚源琼   

  1. 福建师范大学福清分校,泉州出入境检验检疫局,福建省建阳市土肥站
  • 收稿日期:2014-06-10 修回日期:2014-06-10 接受日期:2014-09-05 出版日期:2014-11-20 发布日期:2014-11-20
  • 通讯作者: 汤鸣强
  • 基金资助:
    福建省自然基金项目“海藻酸钠固定化芽孢杆菌TAP-1 有机磷农药降解酶及其酶学性质的表征”(2012J01118)

Effects of Leaf Fertilizer Penshibao on the Pesticide Residue and Activities of Antioxidases in Brassica albograbra Bailey

  • Received:2014-06-10 Revised:2014-06-10 Accepted:2014-09-05 Online:2014-11-20 Published:2014-11-20

摘要: 为探明叶面肥喷施宝对蔬菜农药残留及抗氧化酶活性的效应,以芥蓝(Brassica albograbra)为材料,在室外栽培条件下研究叶面肥喷施宝对芥蓝菜叶片3种杀虫剂(吡虫啉、毒死蜱和高效氯氰菊酯)残留及其超氧歧化酶(SOD)、过氧化氢酶、过氧化物酶(POD)活性的影响。结果表明,与对照相比,喷施宝能促进芥蓝菜吡虫啉、毒死蜱和高效氯氰菊酯残留的降解,但受使用时间与浓度的影响。叶面肥喷施宝能提高高效氯氰菊酯使用后芥蓝菜过氧化氢酶(CAT)与超氧歧化酶(SOD)的活性和毒死蜱胁迫下芥蓝菜CAT的活性,对过氧化物酶(POD)的影响不显著。喷施宝能明显提高吡虫啉胁迫下芥蓝菜POD的活性,对CAT的影响受使用浓度与取样时间的影响,但对SOD的影响不显著。

关键词: 双向电泳, 双向电泳

Abstract: In order to investigate the effect of leaf fertilizer Penshibao on pesticide residue and activities of antioxidant enzyme in vegetables, the study investigated the effect of such fertilizer on three pesticide residue (imidacloprid, chlorpyrifos and beta-cypermethrin) and the activities of superoxidedismutase (SOD), catalase (CAT), peroxidase(POD) in Brassica albograbra under outdoor cultivation conditions. The results showed that, compared with control, the leaf fertilizer Penshibao could promote degradation of imidacloprid, chlorpyrifos and beta- cypermethrin in the vegetable while the extents were affected by the concentration and time of application. Penshibao increased activities of CAT and SOD in Brassica albograbra under the stress of betacypermethrin as well as activities of CAT under the stress of chlorpyrifos while no significant effects of the same fertilizer on activities of POD appeared under the stress of chlorpyrifos. Under the stress of imidacloprid, Penshibao increased activities of POD in Brassica albograbra significantly while its effects on activities of SOD under the same pesticide were not significant.