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中国农学通报

中国农学通报 ›› 2005, Vol. 21 ›› Issue (12): 40-40.

所属专题： 生物技术； 油料作物

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花生DNA分子标记的研究 III 不同限制酶组合AFLP分析效果比较

徐建芝,王传堂   

  • 出版日期:2005-12-05 发布日期:2005-12-05

DNA Molecular Markers in Peanut III．Effects of Different Enzyme Combinations on AFLP Analysis

Xu Jianzhi, Wang Chuantang   

  • Online:2005-12-05 Published:2005-12-05

摘要： 采用了28对EcoR I/Mse I AFLP选择性扩增引物、64对Pst I /Taq I AFLP选择性扩增引物，对作图亲本24-3、B4及其杂种F1进行了分析，统计了两种限制酶组合的扩增总带数、多态性带数及多态性率。秩和检验结果说明，两种限制酶组合AFLP每对引物扩增出的总带数无显著差异，而多态性条带数目以及多态性率均有极显著的差异。证明尝试不同的限制酶组合可望揭示出花生品系材料间更为丰富的遗传变异性。

Abstract: Totally 28 EcoR I/Mse I AFLP selective primer pairs and 64 Pst I /Taq I AFLP selective primer pairs were used in the analysis of 2 peanut mapping parental lines and their F1 hybrid. The NPAR1WAY procedure was conducted using the SAS System for Windows 6.12 package. The results showed significant difference（at 0.01 level）between enzyme combinations in the number of polymorphic bands and polymorphic percentage，and no significant difference in total number of bands，on a per primer pair basis. The study demonstrated that resorting to alternative enzyme combinations may be judicious options in disclosing the genetic variations in the cultivated peanut.