西门塔尔牛耳皮肤成纤维细胞的体外培养

doi:10.11924/j.issn.1000-6850.casb17100021

中国农学通报 ›› 2019, Vol. 35 ›› Issue (3): 47-50.doi: 10.11924/j.issn.1000-6850.casb17100021

所属专题：生物技术；畜牧兽医

西门塔尔牛耳皮肤成纤维细胞的体外培养

詹同彤, 倪和民, 郭勇, 王相国

北京农学院动物科学技术学院

收稿日期:2017-10-10 修回日期:2017-11-13 接受日期:2017-12-21 出版日期:2019-01-28 发布日期:2019-01-28
通讯作者: 王相国
基金资助:
2016 年度科技创新服务能力建设-科技计划重点项目“牛胚胎源IFNτ及其受体体外调节子宫源Integrin αvβ3 差异表达表达的研究”（项目批准号：KZ201610020018）；北京市奶牛产业创新团队(BAIC05-2017)。

In Vitro Culture of Fibroblast Cells from Simmental Ear Tissue

Received:2017-10-10 Revised:2017-11-13 Accepted:2017-12-21 Online:2019-01-28 Published:2019-01-28

摘要/Abstract

摘要： 供体动物细胞体外培养模式的建立是动物克隆研究与生产的必要条件。本试验先利用组织块培养再利用胰酶消化纯化的方法培养西门塔尔牛耳皮肤成纤维细胞,进而为牛体细胞核移植做准备。结果表明,先组织块培养后再消化纯化的方法获得的牛耳皮肤成纤维细胞形态良好,传代后的细胞成活率较高,贴壁良好,且牛耳皮肤细胞的增殖情况符合体外培养细胞正常生长增殖的一般规律。因此,利用组织块培养后再消化纯化是一种获得牛耳皮肤成纤维细胞简单而有效的方法,能够作为牛体细胞核移植技术中供体细胞制备方法。

Abstract: The establishment of donor animal cells in vitro culture model is necessary for animal cloning and production. In order to prepare bovine somatic cell nuclear transfer, the digestion and purification after tissue block culture method were used in vitro culture fibroblast cells in this experiment. The results showed that the fibroblast cell morphology was good, the passaged cell grew well with a high survival rate and good adhesion, and the proliferation of ear fibroblast cells accords with the general rule of cell growth in vitro using the digestion and purification after tissue block culture method. Therefore, tissue culture after digestion of purified bovine ear fibroblast cells is a simple and effective method, which can be used as a method for bovine somatic cell nuclear transfer preparation.

詹同彤,倪和民,郭勇,王相国. 西门塔尔牛耳皮肤成纤维细胞的体外培养[J]. 中国农学通报, 2019, 35(3): 47-50.

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西门塔尔牛耳皮肤成纤维细胞的体外培养

In Vitro Culture of Fibroblast Cells from Simmental Ear Tissue

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