欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2019, Vol. 35 ›› Issue (3): 51-57.doi: 10.11924/j.issn.1000-6850.casb17100054

• 生物技术科学 • 上一篇    下一篇

不同红曲菌株产麦角固醇、色素及桔霉素的比较分析

何 坤1, 周有祥2, 邵彦春1, 陈福生1   

  1. 1.华中农业大学食品科学技术学院;2.湖北省农业科学院农业质量标准与检测技术研究所
  • 收稿日期:2017-10-17 修回日期:2018-12-29 接受日期:2018-02-06 出版日期:2019-01-28 发布日期:2019-01-28
  • 通讯作者: 邵彦春
  • 基金资助:
    湖北省科技支撑计划项目“红曲色素高产菌株的构建与安全性分析”(2015BBA150)。

A Comparative Analysis of the Abilities to Produce Ergosterol, Pigment and Citrinin by Different Monascus Strains

  • Received:2017-10-17 Revised:2018-12-29 Accepted:2018-02-06 Online:2019-01-28 Published:2019-01-28

PDF (PC)

230

摘要： 本研究对实验室购买、收集的9株红曲菌株分泌麦角固醇、色素和桔霉素的能力进行比较分析，并探讨了三者的相关性，旨在为红曲菌资源的深度挖掘提供基础信息。分别采用UPLC-紫外检测法、UV-VIS法和UPLC-荧光检测法测定了9株红曲菌发酵制备红曲样品中麦角固醇、色价和桔霉素的含量；在此基础上，采用统计学分析方法分析了三者之间的相关性。分析表明，不同菌株产麦角固醇、色素和桔霉素的能力不同，其中紫色红曲菌OUT 2011发酵红曲中麦角固醇含量和色价均最高，分别达到(1121.96±55.06) mg/kg和(1058.97±5.04) U，安卡红曲菌AS 3.554发酵红曲中麦角固醇含量和色价均最低，而从毛红曲菌OUT 2137、红色红曲菌AS 3.549，安卡红曲菌AS 3.554、发白红曲菌CICC5017、橙色红曲菌CICC5015发酵的红曲均未检出桔霉素。9株红曲菌发酵产麦角固醇、色素和桔霉素能力各不相同；相关性分析显示菌株分泌麦角固醇和色素的能力具有正相关性，为筛选高产麦角固醇的红曲菌株提供了依据。而桔霉素和麦角固醇及色素之间没有关联性，提示富含色素和麦角固醇的红曲产品需要检测桔霉素，以消除红曲产品的安全隐患。

Abstract: 9Monascus strains stored in the lab were compared to test their abilities to produce ergosterol, pigment and citrinin for further utilizingMonascus strain in the future. UPLC with UV detector, UV-VIS and UPLC with fluorescence detector were adopted to measure ergosterol, pigment and citrinin produced by these 9 strains, and the correlation of these 3 compounds were evaluated by statistical methods. The results revealed that the 9Monascus strains had different abilities to produce ergosterol, pigment and citrinin. Among them, OUT 2011 (M. purpureus ) had the strongest ability to produce ergosterol and pigment, amounting to (1121.96± 55.06) mg/kg and (1058.97 ± 5.04) U, respectively; AS 3.554 (M. anka ) had the lowest ability to produce ergosterol and pigment. No citrinin was detected in red yeast rice made by strains of OUT2137 (M. pilosus ), AS 3.549 (M. ruber ), AS 3.554(M. anka ), CICC5017 (M. albidus ) and CICC5015 (M. aurantiacus ). The production of ergosterol was positively correlated with the roduction of pigment. The putative correlation between ergosterol and pigment laid a foundation for screening strain with high production of ergosterol.