FSHR基因的PCR-RFLP对牛双胎性状的标记分析

摘要/Abstract

摘要： FSHR基因5’端（包括侧翼序列和第一外显子）的限制性内切酶TaqⅠ的PCR-RFLP标记研究发现，在秦川牛中双胎母牛的A基因频率（0.5500）和AA基因型频率（0.5000）分别高于单胎母牛的A基因频率（0.3500）和AA基因型频率（0.3000）；在黑白花奶牛中双胎母牛的B基因频率（0.5937）和BB基因型频率（0.6875）均高于单胎母牛的B基因频率（0.5416）和BB基因型频率（0.4167），也就是FSHR基因的5’端的PCR-RFLP标记在一个品种内单胎母牛和双胎母牛之间有一定趋势，但两个品种的趋势不同。

关键词: 金银花, 金银花, 花期, 有效成分

Abstract: PCR-RFLP in FSHR gene 5’–flanking region with exon 1 digested by restriction endonuclease TaqⅠdemonstrated that the frequencies of allele A (0.5500) and genotype AA (0.5000) in twinning cows were higher than that of allele A (0.3500) and genotype AA (0.3000) in monovular cows for Qinchuan cattle, while The frequencies of allele B (0.5937) and genotype BB(0.6875) in twinning cows were higher than that of allele B (0.5416) and genotype BB (0.4167) in monovular cows for Holstein cattle. Key words: Bovine; Twinning; FSHR gene; PCR-RFLP

雷雪芹,,陈宏, ,徐廷生,袁志发,雷初朝,孙维斌,胡沈荣. FSHR基因的PCR-RFLP对牛双胎性状的标记分析[J]. 中国农学通报, 2003, 19(4): 7-7.

LEI Xue-qin, , CHEN Hong, , XU Ting-sheng , YUAN Zhi-fa, LEI Chu-zhao, SUN Wei-bin, HU Shen-rong. Analysis on Marker on Bovine Twinning trait with PCR-RFLP of FSHR Gene[J]. Chinese Agricultural Science Bulletin, 2003, 19(4): 7-7.

参考文献

1 Gregory K E, Bennett G L，Van Vleck L D，Echternkamp S E and Cundiff L V. Genetic and environmental parameters for ovulation rate, twinning rate, and weight traits in a cattle population selected for twinning [J]. J Anim Sci，1997, 75: 1213-1222
2 Bindon B M. Reproductive biology of Booroola Merino sheep [J]. Aust J Biol Sci, 1984, 37: 163-189.
3 Syrstad O. Inheritance of multiple birth in cattle[J]. Livest Prod Sci，1984, 11: 373-380
4 Karlsen A, Ruane J, Klemetsdal G and Heringstad B. Twinning rate in Norwegian cattle: Frequency, (co) variance components, and genetic trends[J]. J Anim Sci, 2000, 78:15-20
5 雷雪芹，陈宏，袁志发. 牛双胎的研究及应用[J]. 黄牛杂志，2001, 27(6):35－40
6 雷雪芹，陈宏，徐廷生，袁志发. 牛羊多胎性状的分子标记研究[J]. 黄牛杂志, 2002，28(3)：24－27
7 Gregory K E, Echternkamp S E, Dickerson G E, Cundff L V. Twinning in cattle: I. Foundation animals and genetic and environmental effects on twinning rate [J]. J Anim Sci 1990, 68: 1867-1876
8 Echternkamp S E, Gregory K E, Dickerson G E, Cundiff L V. Twinning in cattle: II. Genetic and environmental effects on ovulation rate in pubertal heifers and postpartum cows and the effects of ovulation rate on embryonic survival[J]. J Anim Sci, 1990, 68: 1877-1888
9 Gregory K E, Echternkamp S E, Dickerson G E, Twinning in cattle:Ⅲ.Effects of twinning on dystocia, reproductive traits, calf surviva, calf growth and cow productivity[J]. J Anim Sci, 1990, 68: 3133-3144
10 Van Vleck L D, Gregory K E, Echternkamp S E, Ovulation rate twinning rate in cattle: Heritabilities and genetic correlation [J]. 1991, 69: 3213-3219
11 Fleming J S, Greenwood P J et al. Expression of gonadotrophin subunit genes in sheep that were homozygous carrier and non-carrier of the Booroola fecundity gene FecB [J]. J Reprod Fertil, 1995, 103: 315-321
12 McNatty K P, Hudson N et al. Differences in gonadotrophin concentration and pituitary responsiveness to GnRH between Booroola ewes which were homozygous (FF) and non-carriers (++) of a major gene influencing their ovulation rate [J]. J Reprod Fertil, 1987, 80: 577-588
13 郑亦辉，张德福，马恒东. 湖羊和美利奴羊发情期外周血浆中促性腺激素脉冲分泌的差异 [J]. 中国畜牧杂志，1991，27(2): 23-24。
14 McNatty K P, Hudson N L et al. Gonadotrophin-releasing hormone (GnRH) – inducd gonadotrophin secretion in hypothalamic – pituitary disconnected (HPD-OVX) Booroola ewes [J]. J Reprod Fertil, 1991, 91: 583-592
15 McNatty K P, Hudson N L et al. Plasma concentration of FSH, LH, Thyroid-stimulating hormone and Growth hormone after exogenous stimulation with GnRH in Booroola ewes that are homozygous carrier or non-carriers of FecB gene [J]. J Reprod Fertil, 1994, 102: 177-183
16 Bindon B M et al. Genetic and hormonal factors affecting superovulation [J]. Theriogenology. 1986，25(1): 53-70.
17 Fry R C et al. Induction of ovulation in chronically hypolohysectomized Booroola ewes [J]. J Reprod Fertil, 1988，82: 711
18 魏伍川. 牛羊FHSR基因5’转录启动调控区和部分结构区序列的研究 [D]. 西北农林科技大学博士学位论文， 2000

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