欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2020, Vol. 36 ›› Issue (22): 61-68.doi: 10.11924/j.issn.1000-6850.casb20190800510

所属专题： 园艺

• 林学·园艺·园林 • 上一篇    下一篇

药用植物白及组培苗瓶内复壮研究

席银凯1,2(), 黄衡宇1,3(), 曾彪4()   

  1. 1云南中医药大学/云南省道地濒危中药材繁育与栽培工程技术研究中心,昆明 650500
    2贵州中医药大学,贵阳 550000
    3丽江市古城区秋成种养殖有限公司,云南丽江 674100
    4云南省农业科学院,昆明 650200
  • 收稿日期:2019-08-09 修回日期:2019-11-19 出版日期:2020-08-05 发布日期:2020-07-22
  • 通讯作者: 黄衡宇,曾彪
  • 作者简介:席银凯,女,1993年出生,云南人,硕士研究生,研究方向主要为药用植物学与化学成分研究。通信地址：550025 贵州省贵阳市花溪区党武乡栋青路 贵州中医药大学,E-mail: xyk_xyt@163.com。
  • 基金资助:
    国家自然科学基金“活性氧物质(ROS)调控滇重楼种子萌发成苗的分子机制研究”(81860674);云南省科技厅重点工程技术研究中心项目“云南省道地濒危中药材繁育与栽培工程技术研究中心”(2016DH011)

The Rejuvenation of Medicinal Plant Bletilla striata in Vitro

Xi Yinkai1,2(), Huang Hengyu1,3(), Zeng Biao4()   

  1. 1Yunnan University of Chinese Medicine/Yunnan Breeding and Cultivation Research and Development Center of Endangered and Daodi Traditional Chinese Medicinal Materials, Kunming 650500
    2Guizhou University of Traditional Chinese Medicine, Guiyang 550000
    3Qiucheng Breeding Company Limited, Lijiang Yunnan 674100
    4Yunnan Academy of Agricultural Sciences, Kunming 650200
  • Received:2019-08-09 Revised:2019-11-19 Online:2020-08-05 Published:2020-07-22
  • Contact: Huang Hengyu,Zeng Biao

RichHTML

8

PDF (PC)

254

摘要：

对白及(Bletilla striata)试管苗进行复壮研究,以期解决其羸弱纤细、不利于生根培养及生根后成活率低等问题,同时优化复壮培养条件。在课题组已有的白及增殖及生根培养基配方中,分别添加不同质量浓度植物生长延缓剂多效唑(PP333)和矮壮素(CCC)并改变培养基碳源浓度,以不同继代试管苗为材料,通过单因素和L9(34)正交实验研究不同材料和不同植物生长延缓剂种类及其质量浓度对试管苗复壮、繁殖生长、生根培养及驯化移栽的影响。实验表明,4代苗在MS+活性炭1.0 g/L+香蕉泥50 g/L+ 6-BA 0.5 mg/L+ NAA 0.01 mg/L+ PP333 0.5 mg/L+CCC 0.1 mg/L+蔗糖40 g/L中获得最佳的复壮效果,培养70天后,试管苗基茎直径平均达(0.26±0.06) cm,繁殖系数2.13,假鳞茎发生率100%;复壮后的试管苗在1/2MS+活性炭1.0 g/L+香蕉泥80 g/L+ 6-BA 0.1 mg/L+ NAA 1.0 mg/L+蔗糖15 g/L中培养60天后,可获得具假鳞茎、根粗苗壮的再生植株,生根率100%;再生苗移栽成活率亦达100%。本研究完善了白及体外快繁及复壮条件,建立了高效的植株再生体系。

关键词: 白及, 复壮培养, 植物生长延缓剂, 假鳞茎, 驯化移栽

Abstract:

Since the transplanting seedlings are difficult to adapt to rooting culture and domestication due to the tenuous stems in medium, the rejuvenation research was conducted using test-tube plantlets of Bletilla striata by optimizing the conditions of the medium. Based on the proliferative and rhizogenic formulas established in our previous study, the single factor and L9(34) orthogonal experiment were carried out using different subculture plantlets, different concentrations of plant growth retardant (PP333 and CCC) and carbon source, aiming to investigate the impact of different types and concentrations of plant growth retardant and explants on rejuvenation, multiplication, rooting culture and transplanting. L9(34) orthogonal test exhibited that the optimal rejuvenation medium was MS+ 1.0 g/L AC+ 50 g/L banana puree+ 0.5 mg/L 6-BA+ 0.01 mg/L NAA+ 0.5 mg/L PP333+ 0.1 mg/L CCC+ 40 g/L sucrose for the fourth generation. After 70 d, the diameter of the plantlets’ basal part of stem, the growth coefficient and the occurrence rate was (0.26±0.06) cm, 2.13 and 100%, respectively. The pseudobulbs and roots were sent forth from rejuvenation plantlets after 60 d on the 1/2MS medium containing 1.0 g/L AC, 80 g/L banana puree, 0.1 mg/L 6-BA, 1.0 mg/L NAA and 15 g/L sucrose. The survival rate of transplant seedlings was 100%. High-efficiency regeneration system of B. striata with optimal rejuvenation condition was established.

Key words: Bletilla striata, rejuvenation cultivation, plant growth retardant, pseudobulb, acclimatization transplant

中图分类号: