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中国农学通报

中国农学通报 ›› 2012, Vol. 28 ›› Issue (16): 245-249.doi: 10.11924/j.issn.1000-6850.2011-1968

• 林学 园艺 园林 • 上一篇    下一篇

太子参壮苗生根及驯化移栽技术研究

宋丽莎 林茂 文治瑞 周琨 黄永玉 蔡洪芳   

  • 收稿日期:2011-07-05 修回日期:2012-02-05 出版日期:2012-06-05 发布日期:2012-06-05
  • 基金资助:

    基金项目：贵州省农业科学院院重点项目

Rooting Culture and Training Transplant Technique of Pseudostellaria heterophylla Miq.

  • Received:2011-07-05 Revised:2012-02-05 Online:2012-06-05 Published:2012-06-05

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摘要：

本试验旨在优化太子参壮苗生根和驯化移栽技术。试验以MS为基础培养基，添加NAA、6-BA和马铃薯淀粉，采用L94(3)设计进行壮苗生根试验，不同配比基质进行栽移试验。通过以MS+蔗糖30 g/L为基础培养基，调节pH5.8，添加NAA 0.1、0.3、0.5 mg/L，6-BA 0、0.5、1.0 mg/L和马铃薯淀粉0、10、20 g/L进行研究，当基础培养基+NAA0.3 mg/L+6-BA0 mg/L+马铃薯淀粉10 g/L时诱导生根数最高，平均达26.125；不同移栽基质对太子参移栽成活率的影响较大，采用全腐殖土和腐殖土50%+珍珠岩50%作为移栽基质，成活率分别达97.5%和92.5%。

关键词: 遗传多样性, 遗传多样性

Abstract:

Optimization of seedling rooting and transplanting techniques of domestication of Pseudostellaria heterophylla in this study. Test based on MS culture medium, adding NAA, 6-BA and potato starch, use L94(3) orthogonal test on seedling rooting and transplanting of different substrates. MS culture medium+sucrose 30 g/L as basic culture medium and pH5.8, added NAA 0.1, 0.3, 0.5 mg/L, 6-BA 0, 0.5, 1 mg/L and potato starch 0, 10, 20 g/L were studied, the highest average root 26.125 was induced by the basic culture medium+NAA 0.3 mg/L+6-BA 0 mg/L+potato starch 10 g/L. Different soil substrates are effecting on survival rate of transplanting of Pseudostellaria heterophylla, using humus soil and humus soil 50%+perlite 50% as substrates, the survival rates were 97.5% and 92.5%.