根癌农杆菌介导gna基因对水稻的转化

摘要/Abstract

摘要： 褐飞虱在中国分布很广，近年来已成为南方稻区的主要害虫。大量研究表明,进行抗虫基因分子育种是迅速改良水稻褐飞虱抗性的有效途径。采用农杆菌介导法将来源于大肠杆菌的6-磷酸甘露糖异构酶（PMI）安全选择标记基因和雪花莲凝集素（GNA）抗虫基因构建于同一表达载体上进行遗传转化，获得带有安全标记基因的抗褐飞虱转基因植株。通过PCR扩增、Southern印迹和GUS检测分析表明,PMI标记基因和抗虫GNA基因同时被转入部分植株中并得到了表达，其外缘基因主要以单拷贝的方式插入水稻基因组中。对随机选取的8个转基因单株T0代种子经含潮霉素的培养基培养15d后抗感苗数统计分析表明，绝大多数转基因植株后代符合单基因的遗传分离规律。

Abstract: Brown plant hopper distributes very wide, and it has been the major rice insects in Southern China. Many studies showed that it was economical and effective method to control insects with transgenic breeding. An expression vector was constructed with a safe marker gene, 6-phosphomannose isomerase (PMI), and an insect-resistant gene, Galanthus nivalis agglutinin（GNA）, was used in rice genetic transformation in the present study. Results indicated that the pmi and gna gene have been expressed and transferred into some rice cultivars with PCR analysis, southern blotting and GUS detection, and the gna gene was mainly integrated into rice genome in manner of single copy. Some mature seeds randomly selected from eight transgenic rice plants were cultured 15 days on 1/2 MS medium with 50 mg/L hygromycin, and numbered the resistant and sensitive seedlings. The results indicated that most of the transgenic plants were according with the law of single gene segregation.

张启军,,尹福强,王世全,,李平,,,邹江石,吕川根. 根癌农杆菌介导gna基因对水稻的转化[J]. 中国农学通报, 2006, 22(6): 40-40.

Zhang Qijun,, Yin Fuqiang, Wang Shiquan,, Li Ping,, Zou Jiangshi, Lv Chuangen. Agrobacterium tumefaciens-mediated Transformation of Rice for gna Gene[J]. Chinese Agricultural Science Bulletin, 2006, 22(6): 40-40.

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