欢迎访问《中国农学通报》，
中国农学通报

中国农学通报 ›› 2006, Vol. 22 ›› Issue (6): 45-45.

所属专题： 园艺

• 目次 • 上一篇    下一篇

影响黄瓜直接不定芽诱导的品种等因素研究

金宝燕,苏 华,任华中   

  • 出版日期:2006-06-05 发布日期:2006-06-05

Effects of Cultivar etc on Direct Shoot Induction from Cotyledonary Nodes of Cucumber

Jin Baoyan, Su Hua, Ren Huazhong   

  • Online:2006-06-05 Published:2006-06-05

PDF (PC)

539

摘要： 以黄瓜（Cucumis sativus L.）子叶节为外植体，研究了品种、6-苄氨基嘌呤、外植体大小及硝酸银对黄瓜直接不定芽诱导的影响。结果表明：不同黄瓜品种直接不定芽数量诱导存在显著差异，但长度诱导无显著差异；6-苄氨基嘌呤对直接不定芽数量和长度的诱导作用显著，其中4.0mg/L为数量诱导适宜浓度，其出芽率和每外植体出芽数达到最高，分别为100.0%和11.1，0.5mg/L为长度诱导适宜浓度，其平均芽长12.0mm；外植体的大小对直接不定芽数量和长度均具显著影响，随着子叶的增大诱导直接不定芽的数量和长度增加，其中单片完整子叶为最适宜大小，其出芽率、每外植体出芽数和平均芽长分别达到100%、6.7和17.1mm；硝酸银也对直接不定芽数量诱导影响显著，其中2.0mg/L为适宜浓度，出芽率和每外植体出芽数分别达到97.2%和4.2，但对芽长有抑制作用。

关键词: 组织培养, 组织培养, 光自养生长, 非洲菊

Abstract: Effects of 6-benzylaminopurine concentration, cultivar, explant size and AgNO3 concentration on direct shoot induction were studied by sing cotyledonary nodes as explants of cucumber (Cucumis sativus L.). Results showed different cultivar showed significant difference in the frequency of inducing shoots and number of shoots per explant and showed no difference in shoot length. 4.0mg/L 6-benzylaminopurine was the optimal concentration of shoots quantity inducing which frequency of inducing shoots and number of shoots per explant were 100% and 11.1 respectively. 0.5mg/L 6-benzylaminopurine was the optimal concentration of shoots length inducing which average length of shoots was 12.0mm. Explant size had significantly effects on direct shoots quantity and length induction. When complete cotyledon used as explant, frequency of inducing shoots, number of shoots per explant and average length of shoots could approach to 100%, 6.7 and 17.1mm respectively. 2.0mg/L silver nitrate could significantly increase shoot quantity induction, both frequency of inducing shoots and number of shoots per explant could be as high as 97.2% and 4.2 respectively. But silver nitrate also could restrain shoots elongation.