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中国农学通报 ›› 2019, Vol. 35 ›› Issue (12): 100-106.doi: 10.11924/j.issn.1000-6850.casb18010046

所属专题: 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

西红花组织培养研究进展

王桢, 张永春, 杨柳燕, 蔡友铭, 李青竹   

  1. 上海市农业科学院林木果树研究所
  • 收稿日期:2018-01-08 修回日期:2019-04-09 接受日期:2018-10-17 出版日期:2019-04-26 发布日期:2019-04-26
  • 通讯作者: 蔡友铭
  • 基金资助:
    西红花优质种球脱毒繁育技术成果转化与应用,上海市农委成果转化项目,编号:沪农科转字(2016)第2-4号;上海市花卉产业技术体系建设,市农委产业体系建设专项,编号:沪农科产字(2017)第8号;上海植物种苗组培专业技术服务平台,市科委(研发平台专项),编号:15DZ2291000.

Tissue Culture of Crocus Sativus L.: Research Progress

  • Received:2018-01-08 Revised:2019-04-09 Accepted:2018-10-17 Online:2019-04-26 Published:2019-04-26

摘要: 为了研究人工繁育西红花种球的途径,笔者综述了国内外30多年以来的西红花组织培养研究进展,发现利用不同的外植体都有诱导形成小球茎或完整植株的报道,但是其诱导周期长、诱导率较低,未见进行大规模产业化生产的报道。因此,今后应该着重于研究如何缩短诱导周期,提高诱导率、增殖率,加快大球茎培育进程,并进行花器官和子房的组织培养研究,提高柱头状物诱导率,加强细胞大规模培养技术研究,推动次生代谢产物生产的规模开发。笔者就西红花组织培养的研究成果进行了归纳与综述,并对西红花组培进展进行了展望,以期为中国西红花种球规模化和产业化生产提供参考。

关键词: 光肩星天牛, 光肩星天牛, 植物检疫, 有害生物

Abstract: The paper aims to study the ways of artificial breeding of saffron, we review the research progress on tissue culture of saffron in China and abroad for more than 30 years. It is found that the formation of small bulbs or intact plant could be induced by using different explants, but the induction period is long and the induction rate is low, so no large-scale industrial production is reported. Therefore, in the future, we should focus on how to shorten the induction period, increase the rate of induction and proliferation, and accelerate the process of the large bulb cultivation. In addition, the research should be carried on the tissue culture of flower organs and ovary, the improvement of induction rate of stigma-like structure, and the large-scale culture of cell culture should also be strengthened to promote the scale development of the production of secondary metabolites. The paper summarized the research progress on achievements of tissue culture of saffron, and the future prospects of its in order to provide reference for large scale propagation and industrial production of saffron in China.