4种寄主对光肩星天牛成虫解毒酶活性影响

doi:10.11924/j.issn.1000-6850.casb15120044

中国农学通报 ›› 2016, Vol. 32 ›› Issue (14): 79-83.doi: 10.11924/j.issn.1000-6850.casb15120044

所属专题：生物技术

4种寄主对光肩星天牛成虫解毒酶活性影响

阎雄飞¹,万涛²,刘永华¹

(¹榆林学院生命科学学院,陕西榆林 719000；²重庆市风景园林科学研究院,重庆 401329）

收稿日期:2015-12-08 修回日期:2015-12-22 接受日期:2015-12-25 出版日期:2016-05-17 发布日期:2016-05-17
通讯作者: 阎雄飞
基金资助:
榆林学院重点科研项目“光肩星天牛学习行为研究”(13YK40)；榆林市产学研合作项目平台建设“植物病虫害防治实验室”(2014cxy-11-11)；陕西省教育厅科研计划项目“不同寄主对光肩星天牛生长发育和消化酶活性影响”(2010JK930)。

Four Host Trees Affecting Detoxification Enzymes Activities in Adult Anoplophora glabripennis

Yan Xiongfei¹, Wan Tao², Liu Yonghua¹

(¹College of Life and Science, Yulin University, Yulin Shaanxi 719000;²Chongqing Landscape and Gardening Research Institute, Chongqing 401329)

Received:2015-12-08 Revised:2015-12-22 Accepted:2015-12-25 Online:2016-05-17 Published:2016-05-17

摘要/Abstract

摘要： 为明确光肩星天牛寄主范围扩大的生理生化机制，给该虫制定可持续的控制措施，采用生理生化的研究方法，测定了取食复叶槭、白桦、胡杨和白蜡的光肩星天牛成虫体内5种解毒酶的活性。结果表明：复叶槭和白桦上取食后的天牛成虫羧酸酯酶显著高于在胡杨和白蜡上取食后的天牛成虫(P<0.05)，白桦上取食后的天牛成虫谷胱甘肽S-转移酶活性最高，显著高于复叶槭、胡杨和白蜡上取食的天牛(P<0.05)。在复叶槭上取食后的天牛成虫碱性磷酸酯酶活性最高，显著高于在胡杨和白蜡上取食物的天牛，(P<0.05)。说明寄主树种对光肩星天牛成虫体内的羧酸酯酶、谷胱甘肽S-转移酶和碱性酶活性有明显影响。在4种寄主上取食的天牛成虫类酸性磷酸酯酶活性差异均不显著(P>0.05），多酚氧化酶也得到了类似结果。说明寄主树种对天牛体内酸性磷酸酯酶和多酚氧化酶活性影响不大。

关键词: 赤霉病, 赤霉病, 药剂, 复配剂, 防效, 评价

Abstract: In order to explain physiological and biochemical mechanisms of the increasing host range of Anoplophora glabripennis and develop sustainable control measures, activities of five kinds of detoxification enzymes in A. glabripennis fed on Acer negundo (AN), Betula platyphylla (BP), Populus euphratica (PE), and Fraxinus chinensis (FC) were measured. The detoxification enzymes were carboxylesterase, glutathione S-transferase(GSTs), acid phosphatase, alkaline phosphatase and polyphenol oxidase. The results showed that carboxylesterase activity of beetles fed on AN and BP was significantly higher than those fed on PE and FC (P<0.05). Glutathione S-transferase activity of beetles fed on BP was significantly higher than those fed on other 3 host trees (P<0.05). Alkaline phosphatase activity of beetles fed on AN was significantly higher than those fed on PE and FC (P<0.05). The results indicated that the host trees had significant effect on the activities of carboxylesterase, glutathione S-transferase and alkaline phosphatase. The activities of acid phosphatase of beetles fed on the four kinds of host trees were not significant (P>0.05). The similar results were also found on polyphenol oxidase. These results indicated that the host plant had little effect on activities of acid phosphatase and polyphenol oxidase.

中图分类号:

S763.301

阎雄飞,万涛,刘永华. 4种寄主对光肩星天牛成虫解毒酶活性影响[J]. 中国农学通报, 2016, 32(14): 79-83.

Yan Xiongfei,Wan Tao and Liu Yonghua. Four Host Trees Affecting Detoxification Enzymes Activities in Adult Anoplophora glabripennis[J]. Chinese Agricultural Science Bulletin, 2016, 32(14): 79-83.

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4种寄主对光肩星天牛成虫解毒酶活性影响

Four Host Trees Affecting Detoxification Enzymes Activities in Adult Anoplophora glabripennis

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