5-氮杂胞嘧啶核苷降低小麦成熟胚再生频率

doi:10.11924/j.issn.1000-6850.2020-00006

中国农学通报 ›› 2021, Vol. 37 ›› Issue (3): 26-30.doi: 10.11924/j.issn.1000-6850.2020-00006

所属专题：小麦

5-氮杂胞嘧啶核苷降低小麦成熟胚再生频率

董鲁浩¹(), 李晓慧², 宋语宁², 王贺², 李兴国², 别晓敏²()

¹山东农业大学农学院/作物生物学国家重点实验室,山东泰安 271018
²山东农业大学生命科学学院/作物生物学国家重点实验室,山东泰安 271018

收稿日期:2020-04-06 修回日期:2020-06-01 出版日期:2021-01-25 发布日期:2021-01-26
通讯作者: 别晓敏
作者简介:董鲁浩,男,1983年出生,山东文登人,研究生,研究方向：小麦生物技术育种。通信地址：271018 山东省泰安市泰山区岱宗大街61号山东农业大学农学院,Tel：0538-8241418,E-mail： dongluhao89@163.com。
基金资助:
国家科技重大专项“植物基因组编辑技术研究与育种应用”(2019ZX08010003-002-014);国家自然科学基金委员会重大研究计划集成项目“水稻、玉米和小麦产量共性性状的遗传网络解析”(91935302)

5-azacytidine Reduces Regeneration Frequency of Wheat Mature Embryo

Dong Luhao¹(), Li Xiaohui², Song Yuning², Wang He², Li Xingguo², Bie Xiaomin²()

¹College of Agronomy, State Key Laboratory of Crop Biology/Shandong Agricultural University, Tai’an Shandong 271018
²College of Life Sciences, State Key Laboratory of Crop Biology/Shandong Agricultural University, Tai’an Shandong 271018

Received:2020-04-06 Revised:2020-06-01 Online:2021-01-25 Published:2021-01-26
Contact: Bie Xiaomin

摘要/Abstract

摘要：

为了研究组蛋白乙酰化对小麦再生的影响,以CB037、Fielder和中国春(CS)的成熟胚为外植体,在培养基中添加不同浓度的5-氮杂胞嘧啶核苷(5-azaC),分析了胚性愈伤组织诱导率、愈伤组织分化率和绿芽诱导率。结果表明,添加50 μmol/L 5-azaC,CB037绿芽诱导率为54.72%,与对照79.62%相比,下降水平达到极显著差异;添加10 μmol/L和50 μmol/L 5-azaC,Fielder胚性愈伤组织诱导率分别为39.13%和35.71%,绿芽诱导率分别为26.09%和25.14%,与对照相比,均有显著性差异的下降;CS在50 μmol/L浓度处理时,胚性愈伤组织诱导率为23.89%,与对照30.95%相比,有显著性差异的下降。添加5-azaC降低小麦成熟胚再生频率;随着添加浓度升高,降低效果越明显。5-azaC对小麦再生频率的影响有基因型差异。

关键词: 小麦, 5-氮杂胞嘧啶核苷, 成熟胚, 再生频率, 组织培养

Abstract:

To study the effect of histone acetylation on wheat regeneration, mature embryos of CB037, Fielder and Chinese Spring (CS) were used as explants, different concentrations of 5-azacytidine (5-azaC) were added into the culture medium. The embryonic callus induction rate, callus differentiation rate and green shoot induction rate were analyzed. The results showed that with the addition of 50 μmol/L 5-azaC, the green shoot induction rate of CB037 was 54.72% which reduced significantly compared with the control group of 79.62%. With the addition of 10 μmol/L and 50 μmol/L 5-azaC, the embryonic callus induction rates of Fielder were 39.13% and 35.71% respectively, and the green shoot induction rates were 26.09% and 25.14% respectively, which decreased significantly compared with those of the control. While CS was treated with 50 μmol/L, the embryonic callus induction rate was 23.89% and reached significant difference compared with the control group of 30.95%. Adding 5-azaC could reduce the regeneration frequency of wheat mature embryo; with the increase of additive concentration, the reduction effect becomes more obvious and the effects of 5-azaC on the regeneration frequency are different among genotypes.

Key words: wheat, 5-azacytidine, mature embryo, regeneration frequency, tissue culture

中图分类号:

S512.12

董鲁浩, 李晓慧, 宋语宁, 王贺, 李兴国, 别晓敏. 5-氮杂胞嘧啶核苷降低小麦成熟胚再生频率[J]. 中国农学通报, 2021, 37(3): 26-30.

Dong Luhao, Li Xiaohui, Song Yuning, Wang He, Li Xingguo, Bie Xiaomin. 5-azacytidine Reduces Regeneration Frequency of Wheat Mature Embryo[J]. Chinese Agricultural Science Bulletin, 2021, 37(3): 26-30.

图/表 5

参考文献 25

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培养基	成分
预培养基	MS基本培养基（不含MS维生素）+0.75 g/L MgCl₂+15.0 g/L甘露醇+15.0 g/L山梨醇+5.0 mg/L谷氨酰胺+0.5 g/L水解酪蛋白+12.0 g/L葡萄糖+10 mg/L B₁维生素+1.0 mg/L B₆维生素+1.0 mg/L B₃维生素+2.0 mg/L甘氨酸+39 mg/L乙酰丁香酮+2.0 mg/L dicamba+4.0 mg/L AgNO₃+40.0 mg/L半胱氨酸+100.0 g/L维生素C,pH 5.8,8 g/L琼脂
诱导培养基	MS基本培养基+3.0%蔗糖+2.0 mg/L dicamba+4.0 mg/L AgNO₃,pH 5.8,2.4 g/L植物凝胶
分化培养基	MS基本培养基（不含MS维生素）+10.0 mg/L B₁维生素+1.0 mg/L B₃维生素+1.0 mg/L B₆维生素+2.0 mg/L甘氨酸+5.0 mg/L谷氨酰胺+2.0%蔗糖+0.2 mg/L IAA,pH 5.8,2.4 g/L植物凝胶

培养基	成分
预培养基	MS基本培养基（不含MS维生素）+0.75 g/L MgCl₂+15.0 g/L甘露醇+15.0 g/L山梨醇+5.0 mg/L谷氨酰胺+0.5 g/L水解酪蛋白+12.0 g/L葡萄糖+10 mg/L B₁维生素+1.0 mg/L B₆维生素+1.0 mg/L B₃维生素+2.0 mg/L甘氨酸+39 mg/L乙酰丁香酮+2.0 mg/L dicamba+4.0 mg/L AgNO₃+40.0 mg/L半胱氨酸+100.0 g/L维生素C,pH 5.8,8 g/L琼脂
诱导培养基	MS基本培养基+3.0%蔗糖+2.0 mg/L dicamba+4.0 mg/L AgNO₃,pH 5.8,2.4 g/L植物凝胶
分化培养基	MS基本培养基（不含MS维生素）+10.0 mg/L B₁维生素+1.0 mg/L B₃维生素+1.0 mg/L B₆维生素+2.0 mg/L甘氨酸+5.0 mg/L谷氨酰胺+2.0%蔗糖+0.2 mg/L IAA,pH 5.8,2.4 g/L植物凝胶

5-氮杂胞嘧啶核苷降低小麦成熟胚再生频率

5-azacytidine Reduces Regeneration Frequency of Wheat Mature Embryo

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