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中国农学通报

中国农学通报 ›› 2019, Vol. 35 ›› Issue (26): 80-83.doi: 10.11924/j.issn.1000-6850.casb20190500090

所属专题： 生物技术

• 林学 园艺 园林 • 上一篇    下一篇

利用组织培养技术快速繁殖白木香

都明理1, 徐娇1, 朱楚然1, 王丽红2, 隋春1, 魏建和1   

  1. 1.中国医学科学院药用植物研究所;2.佳木斯大学药学院药学系生药学教研室
  • 收稿日期:2019-05-05 修回日期:2019-06-13 接受日期:2019-07-01 出版日期:2019-09-16 发布日期:2019-09-16
  • 通讯作者: 魏建和
  • 基金资助:
    基金：国家自然科学基金面上项目（81773847）

Rapid Propagation of Aquilaria sinensis by Tissue Culture Technology

  • Received:2019-05-05 Revised:2019-06-13 Accepted:2019-07-01 Online:2019-09-16 Published:2019-09-16

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摘要： 本研究利用组织培养技术，以白木香(Aquilaria sinensis)种子为材料，建立快速繁殖白木香的方法体系。首先进行种子消毒，播种到MS培养基上，然后将白木香无菌幼苗切成带1 个腋芽的茎段，诱导白木香丛生芽；剪下生长健壮的单个芽苗，诱导生根。白木香腋芽诱导分化的最佳培养基为MS+1 mg/L6-BA+0.2 mg/L NAA+0.2 mg/L IBA+30 g/L 蔗糖+5 g/L 植物凝胶，白木香组培苗生根的最佳培养基为MS+30 g/L 蔗糖+5 g/L 植物凝胶。白木香组织培养快速繁殖技术将应用于白木香特异种质的快速大量扩繁中。另外，由于白木香种子随采随种、不易保存的特性，组培快繁技术也可随时为白木香结香机理研究提供遗传背景相对一致的大量新鲜幼嫩的组织材料。

关键词: 甘露聚糖, 甘露聚糖, 甘露聚糖酶, 甘露糖苷酶, 半乳糖苷酶, 协同作用

Abstract: In this study, tissue culture technology was used to establish a rapid propagation system of Aquilaria sinensis with the seeds. Firstly, seeds were sterilized and germinated on MS medium. Then, the sterile seedlings were cut into segments with one potential lateral bud to be induced to grow into cluster buds. Finally, the single growing-up plantlets were cut off for rooting. The best medium for differentiation was MS+ 1 mg/L 6-BA+0.2 mg/L NAA+ 0.2 mg/L IBA+ 30 g/L sucrose+ 5 g/L plant gel, and the best medium for rooting of tissue cultured plantlets was MS+ 30 g/L sucrose+ 5 g/L plant gel. The establishment of rapid propagation system for A. sinensis will be applied to the rapid massive propagation of the special germplasms of A. sinensis. In addition, due to the hard preservation of seeds of A. sinensis, the rapid propagation technology can also provide a large number of fresh and tender tissue materials with relatively identical genetic background for the study of agarwood formation mechanism.