茶花粉提取物抑制α-淀粉酶活性的研究

doi:10.11924/j.issn.1000-6850.casb16030116

中国农学通报 ›› 2016, Vol. 32 ›› Issue (26): 27-32.doi: 10.11924/j.issn.1000-6850.casb16030116

所属专题：生物技术；园艺

茶花粉提取物抑制α-淀粉酶活性的研究

吴忠高¹,倪辉²,杨远帆²,王星¹

(¹北京市蚕业蜂业管理站,北京 100029；²集美大学食品与生物工程学院,福建厦门 361021）

收稿日期:2016-03-15 修回日期:2016-04-22 接受日期:2016-05-31 出版日期:2016-09-21 发布日期:2016-09-21
通讯作者: 杨远帆
基金资助:
农业部、财政部现代农业产业技术体系（蜜蜂）(CARS-45-SYZ1)、北京市科技支撑市委、市政府重点工作“生态涵养区蜂产业科技示范与产业化促进工程建设”(Z101105053710009)。

Inhibition Capability of Camellia Pollen Extract on α-Amylase

Wu Zhonggao¹, Ni Hui², Yang Yuanfan², Wang Xing¹

(¹Beijing Sericulture and Apiculture Management Station, Beijing 100029;²College of Food and Biological Engineering, Jimei University, Xiamen Fujian 361021)

Received:2016-03-15 Revised:2016-04-22 Accepted:2016-05-31 Online:2016-09-21 Published:2016-09-21

摘要/Abstract

摘要： 蜂花粉是一种来源广泛且具有多种生物活性的蜂产品。以茶花粉乙醇提取物为对象，研究蜂花粉对α-淀粉酶的抑制活性。结果表明，茶花粉提取物具有较强α-淀粉酶抑制活性，其半抑制浓度为 8.94 mg/mL，对α-淀粉酶的抑制类型为可逆竞争型抑制，抑制常数为1.31 mg/mL。芦丁与茶花粉提取物复合能提高茶花粉提取物抑制α-淀粉酶活的能力，而没食子酸与茶花粉提取物复合降低了茶花粉提取物对α-淀粉酶的抑制能力；维生素C对花粉提取物抑制α-淀粉酶活的能力有显著的减弱作用，且两者复合表现的负协同率随着维生素C浓度的增大而增大。研究结果为蜂花粉的综合性加工利用及天然α-淀粉酶抑制剂的开发利用提供了科学依据。

关键词: 竹荪, 竹荪, 提取物, 抑菌活性

Abstract: Bee pollen is a kind of bee products that has wide resources and lots of important physiological functions. In this paper, Camellia pollen extract (CPE) prepared by ethanol extraction was investigated for its α-amlyase inhibitory activity. As the results, the pollen extract significantly inhibited the activity of α-amlyase. The half-inhibitory concentration (IC₅₀) was determined to be 8.94 mg/mL. The inhibition belonged to non-competitive reversible inhibition with an inhibition constant (K_i) of 1.31 mg/mL. Lutin and gallic acid respectively affected the inhibitory activity as the positive and negative synergist, however, their concentrations did not display consistent trends on the synergistic rate. The strong synergistic effect of vitamin C on the inhibition of CPE was a negative model, which increased along with the increase of vitamin C concentration. These results provide a theoretical reference for comprehensive utilization of bee pollen and the development of α-amylase inhibitors from nature products.

中图分类号:

s896.4

吴忠高,倪辉,杨远帆,王星. 茶花粉提取物抑制α-淀粉酶活性的研究[J]. 中国农学通报, 2016, 32(26): 27-32.

Wu Zhonggao,Ni Hui,Yang Yuanfan and Wang Xing. Inhibition Capability of Camellia Pollen Extract on α-Amylase[J]. Chinese Agricultural Science Bulletin, 2016, 32(26): 27-32.

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茶花粉提取物抑制α-淀粉酶活性的研究

Inhibition Capability of Camellia Pollen Extract on α-Amylase

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