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中国农学通报 ›› 2020, Vol. 36 ›› Issue (4): 130-134.doi: 10.11924/j.issn.1000-6850.casb18110043

所属专题: 园艺

• 植物保护·农药 • 上一篇    下一篇

芥菜提取物对西瓜枯萎病菌的抑菌活性及抑菌谱测定

刘琳1, 袁仁文1, 张蕊1, 王强1, 马喜玲1, 李珊珊2, 范淑英1()   

  1. 1 江西农业大学农学院,南昌 330045
    2 江西生物科技职业学院,南昌 330200
  • 收稿日期:2018-11-12 修回日期:2019-07-18 出版日期:2020-02-05 发布日期:2020-02-08
  • 通讯作者: 范淑英
  • 作者简介:刘琳,女,1994年出生,江西新余人,硕士研究生,研究方向:植物源杀菌剂的分离纯化及抑菌机制。通信地址:330045 江西省南昌市昌北经济技术开发区志敏大道 江西农业大学农学院,E-mail:1294296160@qq.com。
  • 基金资助:
    国家自然科学基金资助项目“茼蒿抑制西瓜枯萎病菌的物质基础及机理研究”(31360487);江西省自然科学基金资助项目“茼蒿抑制西瓜枯萎病菌的物质基础及机理研究”(20132BAB204016)

Extracts of Brassica juncea: Antibacterial Activity to Watermelon Fusarium Wilt and Antibacterial Spectrum Determination

Liu Lin1, Ruan Renwen1, Zhang Rui1, Wang Qiang1, Ma Xiling1, Li Shanshan2, Fan Shuying1()   

  1. 1 College of Agronomy, Jiangxi Agricultural University, Nanchang 330045
    2 Jiangxi Biotech Vocational College, Nanchang 330200
  • Received:2018-11-12 Revised:2019-07-18 Online:2020-02-05 Published:2020-02-08
  • Contact: Fan Shuying

摘要:

研究芥菜提取物的抑菌活性,为开发芥菜作为杀菌剂应用于农业生产奠定基础,为芥菜资源的合理利用提供理论依据。采用菌丝生长速率法,测定芥菜提取物对西瓜枯萎病菌、水稻纹枯病菌、葡萄座腔菌和烟草黑胫菌等植物病原菌菌丝生长的抑制作用。结果表明:采用不同有机溶剂获得的芥菜提取物对西瓜枯萎病均有一定抑制作用,以95%乙醇提取的抑菌效果最好,其提取物的抑菌率可达90%以上。最小抑菌浓度(MIC)和最小杀菌浓度(MFC)分别为0.5、1.0 g/mL,通过毒力方程的测定,它的半抑菌浓度(EC50)为0.2212 g/mL。通过试验对其他10种植物病原菌的抑菌活性表明:芥菜乙醇提取物对水稻纹枯菌、葡萄座腔菌、烟草黑胫菌、辣椒菌核病菌等4种病原菌的抑菌效果较强,其EC50分别为0.0898、0.1265、0.1679、0.1685 g/mL,对葡萄炭疽病菌的抑制效果最弱,EC50为0.5833 g/mL。芥菜提取物具有很强的抑菌活性,并且具有较广的杀菌谱,值得进一步深入研究。

关键词: 芥菜提取物, 西瓜枯萎病, 植物病原菌, 抑菌活性

Abstract:

To study the antibacterial activity of extracts of Brassica juncea, lay a foundation for the development of B. juncea as a fungicide for agricultural production, and provide a basis for the rational use of B. juncea resources, we tested the antibacterial activities of extracts of B. juncea against Fusarium oxysporum f. sp. niveum, Rhizoctonia solani, Botryosphaeria parv and Phytophthora parasitica var. nicotianae by means of mycelium growth rate method. The results showed that: different organic solvent extracts from B. juncea had certain inhibitory effect on watermelon fusarium wilt; the antibacterial effect of extracting with 95% ethanol was the best, and the antibacterial rate of the extract could reach more than 90%; its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MFC) were 0.5 g/mL and 1.0 g/mL, respectively; through the determination of virulence equation, its half bacteriostatic concentration was 0.2212 g/mL. The antibacterial activity of the other 10 plant phytopathogens showed that: the ethanol extracts of B. juncea had strong antibacterial activity against 4 phytopathogens, such as Rhizoctonia solani, Botryosphaeria parv, Phytophthora parasitica var. nicotianae, and Sclerotinia sclerotiorum with the EC50 0.0898 g/mL, 0.1265 g/mL, 0.1679 g/mL and 0.1685 g/mL, respectively, and the inhibition effect against the Colletorichum gloeosporioides was the weakest, and the EC50 was 0.5833 g/mL. The extracts of B. juncea have strong antifungal activities with broad control spectrum, and it is worth further researching.

Key words: extracts from Brassica juncea, watermelon Fusarium wilt, phytopathogen, antibacterial activity

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