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中国农学通报 ›› 2018, Vol. 34 ›› Issue (35): 105-110.doi: 10.11924/j.issn.1000-6850.casb17090027

• 植物保护 农药 • 上一篇    下一篇

艾纳香抗植物病原菌活性成分研究

王鸿发,胡璇,于福来,王凯,王丹,陈振夏,官玲亮,元超,庞玉新   

  1. 中国热带农业科学院热带作物品种资源研究所 海南儋州,中国热带农业科学院热带作物品种资源研究所 海南儋州,中国热带农业科学院热带作物品种资源研究所 海南儋州,中国热带农业科学院热带作物品种资源研究所 海南儋州,中国热带农业科学院热带作物品种资源研究所 海南儋州,中国热带农业科学院热带作物品种资源研究所 海南儋州,中国热带农业科学院热带作物品种资源研究所 海南儋州,中国热带农业科学院热带作物品种资源研究所 海南儋州,中国热带农业科学院热带作物品种资源研究所 海南儋州
  • 收稿日期:2017-09-06 修回日期:2018-11-22 接受日期:2017-12-22 出版日期:2018-12-16 发布日期:2018-12-16
  • 通讯作者: 元超
  • 基金资助:
    国家自然科学基金“黎药“娜龙”的品种整理及用药规律研究”(81374065);中央级公益性科研院所基本科研业务费专项资金资助项目“重 要南药产业化开发关键技术研究与集成应用”(1630032015039);海南省自然科学基金“茉莉酸甲酯对艾纳香活性成分的影响及调控机制研究” (817263)。

Active Components of Anti Plant Pathogenic Fungi from Blumea balsamifera (L.) DC.

  • Received:2017-09-06 Revised:2018-11-22 Accepted:2017-12-22 Online:2018-12-16 Published:2018-12-16

摘要: 为评价黎药艾纳香在农业病害防治方面的应用前景,利用菌丝生长抑制法和化学成分预试法研究不同艾纳香提取部位的抑菌活性成分。醇提水悬萃取法依次获得艾纳香石油醚萃取物,乙酸乙酯萃取物以及水相部位。供试样品浓度依次为1、0.5、0.25和0.125 mg/mL。结果显示,石油醚提取部位活性最强,在1 mg/mL浓度下对Alternaria solani (ACCC 36023)和Fusarium gramineum (ACCC 36249)的抑制率均达到71%。乙酸乙酯部位对菌株Alternaria solani (ACCC 36023)和Fusarium gramineum (ACCC 36249)也表现出较强的抑制活性,在浓度为1 mg/mL时,抑制率分别达到58%和69%。结合化学成分薄层预试验,推测石油醚部位中的主要抑菌成分为挥发油和萜类成分,乙酸乙酯部位中主要为黄酮类成分。这意味着艾纳香石油醚和乙酸乙酯部位富含抗植物病原菌的萜类和黄酮类活性成分,具有开发为植物源杀菌剂的重要价值。

关键词: 青海湖, 青海湖, 河道演变, 湿地生态系统, 裸鲤

Abstract: To evaluate the application perspective of Blumea balsamifera (L.) DC. on defense of agricultural disease, methods including inhibition of mycelia growth and chemical constituents pre-test were adopted to study its anti pathogenic fungi active components. Three fractions including PE (petroleum ether extract), AE (acetic ether extract) and WE (water extract) afforded according to methods of methanol extraction, water suspension and distribution between water and different organic solvents. Concentrations of test samples were prepared as 1, 0.5, 0.25 and 0.125 mg/mL, individually. Results show that the PE part exhibits potent inhibitory activities against growth of two plant pathogenic fungi, namely Alternaria solani (ACCC 36023) and Fusarium gramineum (ACCC 36249), with inhibitory rates of 71%, respectively, at a concentration of 1 mg/mL. The AE part also shows inhibitory activities against Alternaria solani (ACCC 36023) and Fusarium gramineum (ACCC 36249), with inhibitory rates of 58% and 69%, respectively, at a concentration of 1 mg/mL. Accompanied with thin-layer chromatography chemical pre-test, the main antifungal components of PE were postulated to be volatile oils and terpenes, and the AE part mainly contains flavonoids. This study exhibits the PE and AE parts of Blumea balsamifera (L.) DC. contain abundant anti pathogenic active constituents including terpenes and flavonoids, which indicating great value to exploit Blumea balsamifera (L.) DC. as a potential botanical fungicide.