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中国农学通报

中国农学通报 ›› 2022, Vol. 38 ›› Issue (10): 15-21.doi: 10.11924/j.issn.1000-6850.casb2021-0622

所属专题： 生物技术； 园艺

• 林学·园艺·园林 • 上一篇    下一篇

外源激素和基质对滇油杉种子发芽的影响

王姝茜(), 李莲芳(), 刘畅, 姜若超, 黄孝杰, 合金鑫, 李俞鑫   

  1. 西南林业大学,昆明 650224
  • 收稿日期:2021-06-21 修回日期:2021-09-22 出版日期:2022-04-05 发布日期:2022-05-23
  • 通讯作者: 李莲芳
  • 作者简介:王姝茜,女,1997年出生,四川阆中人,在读硕士研究生,研究方向为森林培育。通信地址：650224 云南昆明盘龙区青云街道白龙寺300号 西南林业大学林学院,E-mail： 847674506@qq.com。
  • 基金资助:
    国家“十三五”重点研发计划-林业资源培育及高效利用技术创新重点专项“油松等速生用材树种高效培育技术研究”(2017YFD0600504-1)

Effects of Exogenous Hormone and Substrate on Seed Germination of Keteleeria evelyniana

WANG Shuqian(), LI Lianfang(), LIU Chang, JIANG Ruochao, HUANG Xiaojie, HE Jinxin, LI Yuxin   

  1. Southwest Forestry University, Kunming 650224
  • Received:2021-06-21 Revised:2021-09-22 Online:2022-04-05 Published:2022-05-23
  • Contact: LI Lianfang

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摘要：

为了解激素种类及其浓度和基质对滇油杉(Keteleeria evelyniana)种子发芽的影响,丰富该树种种子发芽的相关资料。采用L9(34)正交设计,开展激素种类及其浓度与基质不同水平及其组合的实验。滇油杉种子发芽过程中,胚芽和子叶与种皮不分离而幼茎萌蘖新芽生长发育为地上器官,故其种子培育的苗木为萌蘖营养繁殖苗。在室内,滇油杉种子播种后18天开始发芽,延续19天后发芽结束,属易发芽种子类。处理组合的发芽率、发芽势、平均发芽时间和发芽指数分别为18.7%~56.0%、8.3%~27.0%、3.3~6.7天和7.0~18.1粒/天,对照（清水浸种播于壤土）则是13.3%、11.0%、9.0天和2.7粒/天,除发芽势外,实验处理发芽指标极显著高于对照(P<0.01)。基质是影响种子发芽的主导因子,壤土有益于种子发芽;IBA和IAA溶液浸种的种子发芽率极显著高于GA3(P<0.01)。0.45 g/L IBA或0.30 g/L IAA浸种播于壤土可提高种子发芽率和发芽势,缩短发芽时间。

关键词: 滇油杉, 外源激素, 基质, 发芽, 萌蘖苗

Abstract:

To understand the effects of hormone types and concentrations and substrate on seed germination of Keteleeria evelyniana, and enrich relevant information of seed germination of this species, L9(34) orthogonal design was employed to conduct the experiment of hormone types and concentrations and substrate at different levels and treatment combinations (TCs). The results show that in the process of seed germination, the germ and cotyledon do not separate from the seed coat, but the young stem sprouts and the new buds grow and develop into the aboveground organs, so the seedlings cultivated by the seeds of K. evelyniana are vegetative propagation seedlings by sprouting buds. The seeds of K. evelyniana begin to germinate 18 days after sowing and the germination ends after 19 days, so the seeds belong to the category of easily germinated seeds in indoor condition. The germination rates (GRs), germination potentials (GPs), mean germination times (MGTs) and germination indexes (GIs) of the TCs are 18.7%-56.0%, 8.3%-27.0%, 3.3-6.7 d and 7.0-18.1 seed/day, respectively, while those of the control (soaking seeds in clear water and sowing on loam) are 13.3%, 11.0%, 9.0 d and 2.7 seed/day, respectively. Except the GP, the germination parameters of the TCs are significantly higher than those of the control (P<0.01). The substrate is the dominant factor affecting seed germination, the loam is beneficial to seed germination; the GRs of the IBA and IAA solution soaking seeds are significantly higher than that of the GA3 (P<0.01). The results show that 0.45 g/L IBA or 0.30 g/L IAA can improve the GR and GP of the seeds and shorten the germination time.

Key words: Keteleeria evelyniana, exogenous hormone, substrate, germination, sprouting seedling

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