玉米和高粱SSR标记在薏苡中的通用性研究

doi:10.11924/j.issn.1000-6850.casb2022-0152

中国农学通报 ›› 2023, Vol. 39 ›› Issue (5): 33-38.doi: 10.11924/j.issn.1000-6850.casb2022-0152

玉米和高粱SSR标记在薏苡中的通用性研究

付瑜华¹(), 蒙秋伊¹, 尚昆¹, 李秀诗¹, 刘凡值¹, 李祥栋²()

¹ 贵州省农业科学院，贵州省亚热带作物研究所，贵州兴义 562400
² 黔西南州农业林业科学研究院，贵州兴义 562400

收稿日期:2022-03-07 修回日期:2022-06-15 出版日期:2023-02-15 发布日期:2023-02-06
作者简介:
付瑜华，女，1980年出生，河南开封人，副研究员，博士，研究方向：种质资源与分子育种。通信地址：562400 贵州省兴义市峰林东路1号贵州省亚热带作物研究所，Tel：0859-3452853，E-mail：fufu6699@aliyun.com。
基金资助:
国家自然科学基金项目“薏苡仁油含量与相关候选基因的关联分析”(31801435); 黔西南州科技计划项目“优异薏苡种质资源圃建设”(2020-1-02); 贵州省农科院重点科技成果培育项目(黔农科院成培计[2021]02号); “两江一河”热带(亚热带)精品果树产业发展子项(黔农科院精品水果产业专项[2021]01号)

SSR Markers of Maize and Sorghum: The Transferability in Adlay

FU Yuhua¹(), MENG Qiuyi¹, SHANG Kun¹, LI Xiushi¹, LIU Fanzhi¹, LI Xiangdong²()

¹ Guizhou Institute of Subtropical Crops, Guizhou Academy of Agricultural Sciences, Xingyi, Guizhou 562400
² Southwest Guizhou Institute of Agricultural and Forestry Sciences, Xingyi, Guizhou 562400

Received:2022-03-07 Revised:2022-06-15 Online:2023-02-15 Published:2023-02-06

摘要/Abstract

摘要：

旨在分析玉米和高粱SSR标记在其近缘种薏苡中的通用性和多态性，以期筛选可用于薏苡种质资源和遗传学研究的分子标记。以11份薏苡资源为供试材料，利用SSR-PCR扩增和毛细管电泳检测方法，对364对SSR引物进行了测试和筛选。结果表明，364对玉米和高粱来源的标记中有163对能在薏苡中扩增并得到清晰的条带，其中44对标记在薏苡中表现出良好的多态性。高粱SSR的通用性和多态性比例分别为58.02%和30.85%，玉米SSR标记的通用性和多态性比例分别为23.45%和26.47%，高粱SSR标记在薏苡中通用性和多态性更好。44对引物在11份薏苡材料中扩增出110条多态性条带，每对引物扩增出的条带数在1~5条之间，平均为2.5条，PIC为0.15~0.79，其中15对引物的PIC值大于0.5，占全部多态性引物的34.09%。筛选出的通用SSR引物可为薏苡种质资源多样性和分子遗传学研究提供可用的遗传标记，也是高粱、玉米和薏苡3种作物之间比较基因组学研究的有力工具。

关键词: 玉米, 高粱, 薏苡, SSR标记, 通用性

Abstract:

In order to select available molecular makers for adlay germplasm and genetics research, the transferability and polymorphism of SSR markers from maize and sorghum were tested in adlay. 11 adlay germplasms were selected as materials, and then 364 SSR primers from maize and sorghum were screened by SSR-PCR and capillary electrophoresis methods. The results showed that 163 out of the 364 markers were successfully amplified in adlay, among which 44 markers showed well polymorphism. The percentage of transferability and polymorphism of SSR markers from sorghum were 58.02% and 30.85%, respectively, while those from maize were 23.45% and 26.47%, respectively. Sorghum SSR markers exhibited better transferability and polymorphism in adlay. A total of 110 alleles were amplified using the 44 SSR primers in the 11 adlay germplasms, with a number of bands ranging from 1 to 5 (an average of 2.5). PIC ranged from 0.15 to 0.79 and 15 SSR primers had PIC greater than 0.5, accounting for 34.09% of the total polymorphic SSR primers. Therefore, the transferable markers could provide useful genetic markers for germplasm diversity and molecular genetics research in adlay, and they could also be a powerful tool for comparative genomics among sorghum, maize and adlay.

Key words: maize, sorghum, adlay, SSR markers, transferability

付瑜华, 蒙秋伊, 尚昆, 李秀诗, 刘凡值, 李祥栋. 玉米和高粱SSR标记在薏苡中的通用性研究[J]. 中国农学通报, 2023, 39(5): 33-38.

FU Yuhua, MENG Qiuyi, SHANG Kun, LI Xiushi, LIU Fanzhi, LI Xiangdong. SSR Markers of Maize and Sorghum: The Transferability in Adlay[J]. Chinese Agricultural Science Bulletin, 2023, 39(5): 33-38.

图/表 4

参考文献 33

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引物编号	引物名称	片段大小/bp	多态性位点	PIC	引物编号	引物名称	片段大小/bp	多态性位点	PIC
sb008	Txp80	272~290	4	0.612	sb118	xtxp210	200~208	2	0.298
sb016	SB5407	150~152	2	0.165	sb120	xcup47	187~206	3	0.512
sb019	SB5O14	232~257	2	0.298	sb128	xcup48	117~124	3	0.760
sb022	Txp481	212~220	3	0.512	sb132	xtxp258	121~136	3	0.314
sb029	Txp130	208~296	3	0.165	sb140	xcup66	257~318	3	0.512
sb065	Xcup02	401~407	2	0.165	sb149	xcup19	112~118	2	0.397
sb069	Xcup61	254~274	2	0.314	sb160	xtxp115	204~212	2	0.298
sb070	Xcup62	150~159	3	0.430	zm019	umc1432y6	206~242	3	0.165
sb071	Xcup63	102~158	4	0.612	zm021	umc1147y4	168~197	4	0.595
sb075	Xtxp114	109~164	3	0.298	zm028	umc1999y3	208~227	3	0.512
sb087	xcup65	222	1	0.165	zm043	bnlg1484	142~177	3	0.612
sb088	xcup32	182~201	2	0.512	zm044	bnlg1866	112~131	2	0.397
sb089	xcup34	136~166	4	0.314	zm084	phi024	137~183	2	0.165
sb091	xcup40	235~263	3	0.314	zm105	bnlg1305	161~200	2	0.165
sb095	xcup74	221~228	2	0.562	zm135	phi115	295~306	2	0.645
sb096	xcup58	176~200	3	0.612	zm139	phi024	183~191	2	0.298
sb098	xcup01	126~132	2	0.496	zs008	phi308707	121~128	2	0.165
sb102	xcup06	340-347	2	0.397	zs017	phi053	250~257	2	0.298
sb103	xcup27	241~303	5	0.793	zs033	umc1792	261	1	0.165
sb108	xgap36	176~196	2	0.165	zs037	umc1805	165~175	2	0.512
sb113	xcup12	102~116	3	0.165	zs042	phi114	151~157	2	0.397
sb115	xcup17	255	1	0.496	zs050	bnlg1724	123~128	2	0.298

引物编号	引物名称	片段大小/bp	多态性位点	PIC	引物编号	引物名称	片段大小/bp	多态性位点	PIC
sb008	Txp80	272~290	4	0.612	sb118	xtxp210	200~208	2	0.298
sb016	SB5407	150~152	2	0.165	sb120	xcup47	187~206	3	0.512
sb019	SB5O14	232~257	2	0.298	sb128	xcup48	117~124	3	0.760
sb022	Txp481	212~220	3	0.512	sb132	xtxp258	121~136	3	0.314
sb029	Txp130	208~296	3	0.165	sb140	xcup66	257~318	3	0.512
sb065	Xcup02	401~407	2	0.165	sb149	xcup19	112~118	2	0.397
sb069	Xcup61	254~274	2	0.314	sb160	xtxp115	204~212	2	0.298
sb070	Xcup62	150~159	3	0.430	zm019	umc1432y6	206~242	3	0.165
sb071	Xcup63	102~158	4	0.612	zm021	umc1147y4	168~197	4	0.595
sb075	Xtxp114	109~164	3	0.298	zm028	umc1999y3	208~227	3	0.512
sb087	xcup65	222	1	0.165	zm043	bnlg1484	142~177	3	0.612
sb088	xcup32	182~201	2	0.512	zm044	bnlg1866	112~131	2	0.397
sb089	xcup34	136~166	4	0.314	zm084	phi024	137~183	2	0.165
sb091	xcup40	235~263	3	0.314	zm105	bnlg1305	161~200	2	0.165
sb095	xcup74	221~228	2	0.562	zm135	phi115	295~306	2	0.645
sb096	xcup58	176~200	3	0.612	zm139	phi024	183~191	2	0.298
sb098	xcup01	126~132	2	0.496	zs008	phi308707	121~128	2	0.165
sb102	xcup06	340-347	2	0.397	zs017	phi053	250~257	2	0.298
sb103	xcup27	241~303	5	0.793	zs033	umc1792	261	1	0.165
sb108	xgap36	176~196	2	0.165	zs037	umc1805	165~175	2	0.512
sb113	xcup12	102~116	3	0.165	zs042	phi114	151~157	2	0.397
sb115	xcup17	255	1	0.496	zs050	bnlg1724	123~128	2	0.298

玉米和高粱SSR标记在薏苡中的通用性研究

SSR Markers of Maize and Sorghum: The Transferability in Adlay

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电泳检测	高粱		玉米		高粱玉米通用	总数
电泳检测	标准	文献	标准	文献	高粱玉米通用	总数
SSR标记	40	122	40	105	57	364
琼脂糖电泳检测阳性SSR标记	12	82	11	23	35	163
毛细管电泳检测具有多态性的SSR标记	5	24	3	6	6	44

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