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中国农学通报 ›› 2006, Vol. 22 ›› Issue (8): 88-88.

所属专题: 生物技术

• 目次 • 上一篇    下一篇

组织培养技术筛选香石竹低玻璃化无性系初报

黄宇翔,吴祖建,柯 昉,刘金燕   

  • 出版日期:2006-08-05 发布日期:2006-08-05

In Vitro Selection for Carnation Clone Against Vitrification

Huang Yuxiang, Wu Zujian, Ke fang, Liu Jinyan   

  • Online:2006-08-05 Published:2006-08-05

摘要: 采用离体培养的方法,对香石竹红色品种‘多明格’的茎尖培养试管苗进行连续多代剔除玻璃苗和畸形苗的筛选,继代培养27代后获得了玻璃化率稳定在8%左右的低玻璃化无性系。该无性系经10代的增殖培养,定植到大田后仍然保持母本的优良性状,无明显的有害变异。

关键词: 玉米, 玉米, 种质, 78599, 利用和改良

Abstract: Plantlets in vitro of ‘Domingo’, one of the red-flowering carnation breeds, were subcultured in vitro. They were selected for obtaining a clone, whose vitrification rate could be controlled less than 10%, by way of progressive subculture. After 27 times of subculture, such a clone was obtained. After 10 times of multiplying culture, the clone could keep the traits of its original one, and no harmful variation was observed, when they grew in a field.

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