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中国农学通报

中国农学通报 ›› 2024, Vol. 40 ›› Issue (3): 112-119.doi: 10.11924/j.issn.1000-6850.casb2023-0081

• 植物保护·农药 • 上一篇    下一篇

甘薯疮痂病菌的生物学特性和产孢方法研究

张新新1,2(), 黄雍1,3, 陈景益1, 邹宏达1, 罗忠霞1, 王章英1, 黄立飞1()   

  1. 1 广东省农业科学院作物研究所，广东省农作物遗传改良重点实验室，广州 510640
    2 博罗县农业科学研究所，广东惠州 516139
    3 仲恺农业工程学院农业与生物学院，广州 510225
  • 收稿日期:2023-01-31 修回日期:2023-05-15 出版日期:2024-01-17 发布日期:2024-01-17
  • 通讯作者:
    黄立飞，男，1979年出生，山西左权人，研究员，博士，主要从事甘薯抗病育种研究。通信地址：510640 广东省广州市天河区金颖路18号作物研究所，Tel：020-85514242，E-mail：。
  • 作者简介:

    张新新，女，1987年出生，河北唐山人，助理研究员，硕士，研究方向：甘薯抗病育种。通信地址：516139 广东省惠州市博罗县湖镇镇下村农场航天基地办公室，Tel：0752-6657086，E-mail：。

  • 基金资助:
    国家甘薯产业技术体系(CARS-10); 广东省重点领域研发计划项目“甘薯优质鲜食型新品种培育及健康种苗技术集成与应用”(2020B020219001); 广东省甘薯马铃薯产业技术体系创新团队项目“病虫害防控岗位”(2023KJ111); 广东省驻镇帮镇扶村农村科技特派员项目“甘薯病虫害绿色防控技术推广应用”(KTP20210313)

Elsinoe batatas Causing Sweetpotato Scab: Biological Characteristics and Conidia Production

ZHANG Xinxin1,2(), HUANG Yong1,3, CHEN Jingyi1, ZOU Hongda1, LUO Zhongxia1, WANG Zhangying1, HUANG Lifei1()   

  1. 1 Crop Research Institute, Guangdong Academy of Agricultural Sciences/Key Laboratory of Crop Genetic Improvement of Guangdong Province, Guangzhou 510640
    2 Boluo County Agricultural Science Research Institute, Huizhou, Guangdong 516139
    3 College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou 510225
  • Received:2023-01-31 Revised:2023-05-15 Published-:2024-01-17 Online:2024-01-17

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摘要：

探究甘薯疮痂病菌(Elsinoe batatas)的生物学特性，筛选疮痂病菌在人工培养基上快速、大量地产生分生孢子的方法，为甘薯抗疮痂病育种提供依据。以疮痂病病原菌菌株CRI-CJ2为研究对象，采用菌丝生长速率法测定在不同培养基、pH和光照处理条件下的菌落生长直径，利用凹玻片悬滴法测定在不同温度、pH和光照处理条件下的分生孢子萌发率，比较Fries液体培养法、摇菌法和菌丝涂布法等3种方法的诱导产孢效果。结果表明，最适菌丝生长的培养基为PDA，pH 6~7，致死温度为48℃、10 min，光照对菌丝生长无显著影响，但连续光照使菌落呈鲜红色；分生孢子萌发最适pH 5，最适温度为25℃，致死温度为48℃、10 min，光照对分生孢子萌发影响不显著；采用菌丝涂布法可使疮痂病菌在培养第12天分生孢子产孢量达7.09×107个/mL，分生孢子萌发率为24%，产孢量和孢子活力均高于摇菌法，采用Fries液体培养法未观察到分生孢子及产孢结构。可见，选用适宜的培养基、pH能促进菌丝体生长，菌丝涂布法具有产孢快、产孢量大的优点，是一种适合甘薯疮痂病菌分生孢子诱生的方法。

关键词: 甘薯疮痂病, Elsinoe batatas, 生物学特性, 产孢, 菌丝涂布法

Abstract:

The biological characteristics of the pathogen of sweetpotato scab (Elsinoe batatas) were studied and the methods of producing a large number of conidia in a short time by E. batatas on artificial medium were screened to provide the basis for resistance breeding of sweetpotato scab. Taking the E. batatas isolate CRI-CJ2 as research object, the mycelial growth rate method was utilized to measure the colonial diameter under different medium, pH values and illumination conditions. The concave slide pendent drop method was used to study the conidial germination rate under different temperature, pH values and illumination conditions, and the effects of the Fries liquid culture method, shaking method and mycelium coating method for inducing conidia production of E. batatas were compared. The results showed that the optimal medium for mycelial growth was PDA, the optimum pH value was 6-7, and the lethal temperature was 48℃ for 10 min. No significant differences were found in the mycelial growth between different illuminations, but the colour of colony turned bright red under continuous illumination condition. The optimum pH value for conidial germination was 5, the best temperature was 25℃, and the conidia could not germinate above 48℃ for 10 min. Illumination showed little effect on conidial germination. The conidial quantity of E. batatas was 7.09×107 conidia/mL after 12 days of incubation by the mycelium coating method and the conidial germination rate was 24% after incubation for 24 hours, and the quantity and viability were higher than that by the shaking method. No conidia or sporogenous structure were observed by the Fries liquid culture method. Therefore, selecting appropriate medium and pH value could promote mycelial growth, and the mycelium coating method had the advantages of rapid sporulation and high conidial quantity and was a suitable method for E. batatas conidia induction.

Key words: sweetpotato scab, Elsinoe batatas, biological characteristics, conidia production, mycelium coating method