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中国农学通报

中国农学通报 ›› 2024, Vol. 40 ›› Issue (3): 120-127.doi: 10.11924/j.issn.1000-6850.casb2023-0147

• 植物保护·农药 • 上一篇    下一篇

不同浓度镉处理对耐盐水稻生长及斜纹夜蛾酶活的影响

徐进1,2(), 谭建彬1, 邓晓瑜1, 梁锦炀1, 师沛琼1,2(), 周鸿凯1,2   

  1. 1 广东海洋大学滨海农业学院，广东湛江 524088
    2 国家耐盐碱水稻技术创新中心华南中心，广东湛江 524088
  • 收稿日期:2023-02-28 修回日期:2023-05-24 出版日期:2024-01-17 发布日期:2024-01-17
  • 通讯作者:
    师沛琼，女，1989年出生，甘肃会宁人，讲师，博士，主要从事昆虫分子生态学研究。通信地址：524088 广东湛江麻章区海大路1号 广东海洋大学滨海农业学院，Tel：0759-2383247，E-mail：。
  • 作者简介:

    徐进，男，1984年出生，山东济南人，讲师，博士，主要从事害虫综合防治。通信地址：524088 广东湛江麻章区海大路1号 广东海洋大学滨海农业学院，Tel：0759-2383247，E-mail：。

  • 基金资助:
    广东省重点领域研发计划项目“耐盐碱水稻重要功能基因挖掘及品种创新与应用”(2020B020219004); 广东海洋大学科研启动经费资助项目“濒海环境外源生物胁迫的果蔬害虫免疫动态调控机理研究”(R19029); “内共生菌对媒介昆虫适生性的调节机制”(R19025); 湛江市科技计划竞争性项目“特色香型海水稻新品种培育及其产业化示范”(2019A03005)

Effects of Different Concentrations of Cadmium on Growth of Salt-Tolerant Rice and Enzyme Activity of Common Cutworm Spodoptera litura (Lepidoptera: Noctuidae)

XU Jin1,2(), TAN Jianbin1, DENG Xiaoyu1, LIANG Jinyang1, SHI Peiqiong1,2(), ZHOU Hongkai1,2   

  1. 1 College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, Guangdong 524088
    2 South China Branch of National Saline-Alkali Tolerant Rice Technology Innovation Center, Zhanjiang, Guangdong 524088
  • Received:2023-02-28 Revised:2023-05-24 Published-:2024-01-17 Online:2024-01-17

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摘要：

为了研究不同浓度的镉胁迫对耐盐水稻生长及昆虫解毒酶的影响，以耐盐水稻品种‘海红-12’为材料，用不同浓度(1.5、3、6、15 mg/kg)的氯化镉溶液分别处理4、7、12、17、22 d，测定了不同浓度、不同时间镉处理下耐盐水稻幼苗株高、根长、叶绿素含量以及以处理水稻植株为食的斜纹夜蛾幼虫乙酰胆碱酯酶和羧酸酯酶的活性。结果表明，镉胁迫下，耐盐水稻株高、根长受镉浓度和时间效应的双重影响，较低浓度(1.5、3 mg/kg)镉溶液对水稻生长的抑制作用在处理期间间歇性呈现，较高浓度(6、15 mg/kg)镉溶液的抑制作用则持续出现。随着镉处理浓度的升高，耐盐水稻的叶绿素含量随时间的推移持续降低。取食不同浓度镉处理水稻幼苗的斜纹夜蛾幼虫，乙酰胆碱酯酶活性随镉处理浓度的升高而降低，但羧酸酯酶活性升高。镉溶液抑制耐盐水稻幼苗的生长，且抑制作用随镉溶液浓度的增大而增强，取食受镉处理的耐盐水稻幼苗，植食性害虫体内解毒酶活性产生不同趋势的变化。研究结果可为优化耐盐水稻种植栽培条件提供数据支撑，为进一步研究耐盐水稻-昆虫对重金属富集的反应机制提供理论依据。

关键词: 镉胁迫, 耐盐水稻, 生长发育, 斜纹夜蛾, 酶活性

Abstract:

To investigate the impact of varying concentrations of cadmium on the growth of salt-tolerant rice and enzyme activity in insects, the salt-tolerant rice variety ‘Haihong-12’ was treated with cadmium chloride solution of different concentrations (1.5, 3, 6, 15 mg/kg) for 4, 7, 12, 17, 22 days, respectively, the plant height, root length, chlorophyll content of salt-tolerant rice seedlings and the activities of acetyl cholinesterase and carboxyl esterase of Spodoptera litura larvae fed on treated rice plants were measured under different concentrations and different time of cadmium treatment. The results indicate that the growth of salt-tolerant rice, including plant height and root length, is influenced by both cadmium concentration and time under cadmium stress. The inhibitory effect of a low concentration (1.5, 3 mg/kg) cadmium solution on rice growth was observed intermittently during the treatment period, whereas the inhibitory effect of a high concentration (6, 15 mg/kg) cadmium solution persisted. The chlorophyll content of salt-tolerant rice and the acetyl cholinesterase activity of Spodoptera litura larvae decreased with increasing cadmium concentration, while the carboxyesterase activity of Spodoptera litura larvae increased. The growth of salt-tolerant rice seedlings was impeded by cadmium solution, and the inhibitory effects were intensified with increasing concentrations of cadmium solution. Phytophagous pests exhibited varying trends in detoxification enzyme activities when fed on salt-tolerant rice seedlings treated with cadmium. The results could provide supportive data for optimizing the cultivation conditions of salt-tolerant rice and provide a theoretical basis for further research on the reaction mechanism of heavy metal enrichment in salt-tolerant rice-insect interaction.

Key words: cadmium stress, salt-tolerant rice, growth condition, Spodoptera litura, enzyme activity