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中国农学通报

中国农学通报 ›› 2026, Vol. 42 ›› Issue (5): 117-127.doi: 10.11924/j.issn.1000-6850.casb2025-0702

• 资源·环境·生态·土壤·气象 • 上一篇    下一篇

酿酒葡萄根围土壤固碳细菌群落结构及固碳微生物筛选

杨偲淇1(), 薛婷婷1,2,3, 赵欣茹1, 郜磊1, 曹凌霄1, 赵智鹏1, 张亮1,2,3()   

  1. 1 宁夏大学葡萄酒与园艺学院， 银川 750021
    2 葡萄与葡萄酒教育部工程研究中心， 银川 750021
    3 宁夏葡萄与葡萄酒工程技术研究中心， 银川 750021
  • 收稿日期:2025-08-21 修回日期:2025-10-11 出版日期:2026-03-18 发布日期:2026-03-18
  • 通讯作者:
    张亮，男，1991年出生，甘肃天水人，讲师，博士，主要从事葡萄智能化栽培与土壤微生态方面的研究工作。通信地址：750021 宁夏回族自治区银川市西夏区怀远路文萃北街215号 宁夏大学，E-mail：。
  • 作者简介:

    杨偲淇，女，2000年出生，浙江嘉兴人，在读硕士，研究方向：酿酒葡萄生态栽培。通信地址：750021 宁夏回族自治区银川市西夏区怀远路文萃北街215号 宁夏大学，E-mail：。

  • 基金资助:
    国家自然科学基金（地区基金）“贺兰山东麓保护性耕作葡萄园土壤团聚体有机碳固存机制”(32360804); 宁夏回族自治区重点研发计划项目“贺兰山东麓酿酒葡萄水肥耦合及水分养分精准调控关键技术研究与示范”(2024BBF02003)

Structure of Carbon Sequestration Bacterial Community and Screening of Carbon Sequestration Microorganisms in Rhizosphere Soil of Wine Grapes

YANG Siqi1(), XUE Tingting1,2,3, ZHAO Xinru1, GAO Lei1, CAO Lingxiao1, ZHAO Zhipeng1, ZHANG Liang1,2,3()   

  1. 1 College of Enology and Horticulture, Ningxia University, Yinchuan 750021
    2 Engineering Research Center of Grape and Wine, Ministry of Education, Yinchuan 750021
    3 Ningxia Grape and Wine Engineering Technology Center, , Yinchuan 750021
  • Received:2025-08-21 Revised:2025-10-11 Published:2026-03-18 Online:2026-03-18

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摘要：

本研究旨在探究葡萄园土壤固碳微生物及其碳源利用特征，为提高酿酒葡萄园土壤有机质提供依据。以‘赤霞珠’葡萄根围土壤为试材，采用高通量测序、无机无碳培养基筛选及Biology-ECO微孔板法，分析固碳细菌群落结构及其碳源利用效率。4个‘赤霞珠’葡萄园土壤优势菌属为慢生根瘤菌属、诺卡氏菌属、中慢生根瘤菌属、固氮螺菌属、红长命菌属。有机管理的博纳佰馥酒庄土壤固碳细菌丰富度最高，Chao1指数较贺东庄园、甘土酒庄分别高29.70%和46.10%，红长命菌属为关键固碳功能菌，占17.24%。本研究分离出的三株固碳功能菌株均偏好碳水化合物与氨基酸类碳源。本研究证实，酿酒葡萄园的固碳细菌群落具有较高物种丰富度，不同酒庄间的固碳细菌群落结构差异显著，有机与精细管理可有效提升其丰度与功能。

关键词: 酿酒葡萄园, 根围土壤, 土壤固碳微生物, 碳源利用, Biology-ECO

Abstract:

This research aims to investigate carbon sequestration microorganisms in vineyard soils and their characteristics of carbon source utilization, in order to provide a basis for enhancing soil organic matter in vineyard systems. Using the rhizosphere soil of 'Cabernet Sauvignon' grapevines as experimental material, this study employed high-throughput sequencing, screening with inorganic carbon-free media, and the Biology-ECO microplate method to analyze the community structure of carbon sequestration microorganisms and their efficiency in carbon source utilization. The dominant bacterial genera in the soils of the four 'Cabernet Sauvignon' vineyards were Bradyrhizobium, Nocardia, Mesorhizobium, Azospirillum, and Rubrivivax. Among them, the organically managed Bonabaifu Vineyard exhibited the highest richness of carbon sequestration microorganisms, with its Chao1 index being 29.70% higher than that of Hedon Vineyard and 46.10% higher than that of Gantu Vineyard. In addition, the genus Rubrivivax was identified as the key functional genus for carbon sequestration, accounting for 17.24%. The three isolated strains of carbon sequestration microorganisms all showed a preference for carbohydrate and amino acid carbon sources. This study confirmed that the carbon sequestration microorganisms in vineyards possessed relatively high species richness, and their community structures differed significantly among different vineyards. Organic and precision management practices can effectively enhance the abundance and functionality of these communities.

Key words: vineyard, rhizosphere soil, soil carbon sequestration microorganisms, carbon source utilization, Biology-ECO