关键词: 苹果酸-乳酸发酵, 苹果酸-乳酸发酵, 固定化细胞, Leuconostoc oenos 31DH

Abstract: The friable embryogenic callus with small grains and without proembryo formation was screened in the process of continuous subcultures for 4~6 times on the medium with low content of sucrose from embryogenic cultures induced from immature embryos in litchi (Litchi chinensis Soon.). The well-scattered embryogenic cell suspensions could be rapidly established from the friable embryogenic callus in 2 liquid initial MS media respectively supplemented with 2,4-D 2 mg/L or 2,4-D 2 mg/L, KT1 mg/L and AgNO3 5 mg/L shaking at 100~120 r/min within 10-14 days. Long-term maintenance of the embryogenic cell suspensions was achieved via alternative cultures in the 2 revised initial medium only with half-strength of phytohormones or through the method of periodical in-turn liquid-solid culture. The embryogenic suspension cells formed somatic embryos at the frequency of over 10,000 embryoids/g FW on the solid MS medium amended with NAA 0.1 mg/L, KT or Ze 5 mg/L, sucrose 50 g/L, agar 10g/L and myo-inositol 100 mg/L within 25~40 days. Normal somatic embryos were converted into plantlets by over 75% after maturation.