植物有效利用磷素营养有机酸代谢途径基因工程研究进展

摘要/Abstract

摘要： 随着现代农业的发展，不断提高土壤磷素营养的有效利用率，对促进农业生产的持续发展具有重要的现实意义。在酸碱性土壤中，磷主要以植物难以吸收利用的难溶性二价或三价阳离子的形式存在。植物增强吸收土壤中难溶性磷素的分子机制是通过改变植物体内有机酸代谢途径，分泌较多的有机酸螯合不溶性磷化合物中的金属离子来增强对磷的吸收。就通过遗传操作在植物体内过量表达或异位表达柠檬酸合成酶、苹果酸脱氢酶基因对促进植物磷吸收作一简要综述。

关键词: 东北大豆, 东北大豆, 蛋白质, 功能特性

Abstract: With development of the modern agriculture，improvement of phosphorus utilization efficiency is very important for promoting sustainable agriculture development. In acid and alkali soil, there are mainly indissoluble bivalent or trivalent phosphates, which cannot be utilized by plants. The molecular mechanism which the plant strongly utilizes the soil phosphorus is excreting more organic acid by altering organic acid metabolic pathway. This paper will make a brief summary on genetic engineering in the over-expression of citrate synthase and malate dehydrogenase in transgenic plants to improve the utilization of insoluble phosphorus.

中图分类号:

赵玥,陈丽梅,李昆志,玉永雄. 植物有效利用磷素营养有机酸代谢途径基因工程研究进展[J]. 中国农学通报, 2007, 23(1): 33-033.

Zhao Yue, Chen Limei, Li Kunzhi, Yu Yongxiong. Genetic Engineering of Organic Acid Metabolic Pathway for Utilizing Phosphorus Nutrition Efficiently by Plants[J]. Chinese Agricultural Science Bulletin, 2007, 23(1): 33-033.

参考文献

[1] Tayor,G.J. Current views of the aluminum stress response: The physiological basis of tolerance. Curr Top Plant Biochem Physiol.1991,10: 57-93.
[2] Devive,T.E.,Bouton,J.H.,Mabrahtu,T.,Legume genetics and breeding for stress tolerance and nutrient efficiency.In VC Baligar,RR Duncan,eds,Crops as Enhancers of Nutrient Use.Academic Press,Boston,1990, 211-252.
[3] Tesfaye,M.,Temple,S.J.,Allan,D.L.,et al.Overexpression of Malate Dehydrogenase in Transgenic Alfalfa Enhances Organic Acid Synthesis and Confers Tolerance to Aluminum. Plant Physiol.2001, 127: 1836-1844.
[4] Bar-yosef, B.In Plant roots, the hidden half. eds Waisel, Y., Eschel, A., Kafkafi V. Marcel Dekker, New York, NY,1991:529-557.
[5] López-Bucio J., Octavio de la Vega M., Guevara-García A, et al. Enhanced phosphorus uptake in transgenic tobacco plants that overproduce citrate.Nature Biotechnology.2000:18:450-453.
[6] Holford,I.C.R.Soil phosphorus: its measurements, and its uptake by plants. Aust. J. Soil. Res. 1997, 35:227-239.
[7] Hoffland,E.,Findenegg,G.R., Nelemans,J.A. Solubilization of rock phosphate by rape.2.Local root exudation of organic acids as a response to P starvation.Plant soil 1989,113:161-165.
[8] Dinkelaker,B.,Romheld,B., Marshner,H. Citric acid excretion and precipitation of calcium citrate in the rhizosphere of white lupine(lupinus albus L.).Plant Cell Environ 1989,12:285-292.
[9] Ando,J. Future of phosphorus resources and suggested direction for Japan.Jpn.J.Soil.Sci.Plant Nutr 1983, 54:164-169.
[10] Schachtman,D.P.,Reid,R.J. Ayling, S. M. Phosphorus Uptake by Plants: From Soil to Cell Plant Physiol 1998,116:447-453.
[11] Johnson,J.F., Vance,C.P., Allan,D.L. Phosphorous stress induced proteoid roots show altered metabolism in Lupinus albus.Plant Physiol 1994,104:657-665.
[12] Lipton,D.S.,Blanchar,R.W., Blevins,D.G. citrate,malate,andsuccinate concentration in exudates from P-sufficient and P-stressed Medicago sativa L. seedlings.Plant Physiol. 1987, 85:315-317.
[13] Gardner，W.k.,Parbery, D.G.,and Barber,D.A. Proteoid root morphology and function in Lupinus albus.Plant Soil 1981, 60:143-147.
[14] Hoffland,E.,Nelemans J.A., Findenegg,G.R. Origin of organic acids exuded by roots of phosphorus-stressed rape (Brassica napus)plants.In Plant Nutrition Physiology and Apprications. 1990:179-184.
[15] Johnson,J.F.,Allan,D.L.,Vance,C.P.,etal. Rootcarbon-dioxide fixation by phosphorus-deficient Lupinus albus:contribution to organic acid exudation by proteoid roots.Plant Physiol ,1996,112:19-30.
[16] 沈同,王镜岩,赵邦悌,等.生物化学(第二版)下册.北京:高等教育出版社,2000:91-100.
[17] Koyama,H., Takita, E., Kawamura, A.,et al. Over Expression of Mitochondrial Citrate Synthase Gene Improves the Growth of Carrot Cells in Al-Phosphate Medium.Plant Cell Physiol, 1999,40(5):482-488.
[18] Koyama,H., Kawamura, A., Kihara1,T.,et al. Overexpression of Mitochondrial Citrate Synthase in Arabidopsis thaliana Improved Growth on a Phosphorus-Limited Soil. Plant and Cell Physiology, 2000,41(9):1030-1037.
[19] Anoop, V. M., Basu U., McCammon M. T., et al. Modulation of Citrate Metabolism Alters Aluminum Tolerance in Yeast and Transgenic Canola Overexpressing a Mitochondrial Citrate Synthase Plant Physiology, 2003,132:2205-2217.
[20] de la Fuente, J.M., Ramirez-Rodrigez, V.,Cabrera-Ponce,J.L.,et al. Aluminum tolerance in transgenic plants by alteration in citrate synthesis.Science 1997,276:1566-1568.
[21] Kruse,A.,Fieuw,S.,Heineke,D.,et al. Antisense inhibition of cytosolic NADP-dependent isocitrate dehydrogenase in transgenic potato plants.Planta 1998,205:82-91.
[22] 王忠,王三根,李合生,等.植物生理学(第一版) 北京:中国农业出版社,2002:157-164.
[23] Miller,S.S.,Driscoll,B.T., Gregerson, R.G., et al. Alfalfa Mlatedehydrogenase(MDH):molecular clonging and characterization of five different forms reveals a unique nodule-enhanced MDH, Plant J 1998,15:173-184.
[24] Mauseth, J. D,Botany, An Introduction to Plant Biology .Florida:Saunders College Publishing , 1991:272-296.
[25] 胡建广,赵相山,刘军,等.玉米苹果酸脱氢酶基因的分离与结构分析.植物学报,1999,41(1):40-44.
[26] 崔小英,崔衍波,邓伟,等.超量表达苹果酸脱氢酶基因提高苜蓿对铝毒的抗受性.分子植物育种,2004,2(5):621-626.
[27] Fox,T.R., Comerford, N.B., McFee, W.W. Phosphorous and aluminum release from a spodic horizon mediated by organic acids. Soil Sci Soc Am J,1990,54: 1763-1767.
[28] Pao S. S., Paulsen I. T. and Saier M.H. Major facilitator superfamily. Microbiol Mol Biol Rev, 1998, 62:1-34.