49份山西水地小麦品系的HMW-GS组成及品质分析

doi:10.11924/j.issn.1000-6850.casb19020037

摘要/Abstract

摘要：

为了解在育种过程中各品系材料的品质状况,采用SDS-PAGE技术对选取的49份品系材料高分子量麦谷蛋白亚基的组成进行分析。结果表明：共发现8种等位变异和11种亚基组合类型。其中,Glu-A1位点出现2种亚基类型,以亚基1(83.67%)为主。Glu-B1位点出现4种亚基类型,以亚基14+15(42.86%)为主。Glu-D1位点出现2种亚基类型,亚基2+12(71.43%)和亚基5+10(28.57%)。对49份材料进行品质性状测定,并与各个亚基间做相关性分析。Glu-A1位点,亚基1的各个指标均高于亚基Null的指标。Glu-D1位点,亚基5+10的品质指标也高于亚基2+12。Glu-B1位点4种亚基在各个品质指标间均存在显著差异,亚基7+8对蛋白质含量、容重以及湿面筋的贡献最高,亚基17+18对沉淀值和最大抗延阻力的贡献最高,亚基7+9具有最长的稳定时间。

关键词: 小麦, 高分子量麦谷蛋白, 品质, 蛋白质含量, 容重, 稳定时间

Abstract:

To study the quality of wheat strains during breeding process, we analyzed the HMW-GS composition of 49 wheat strains by using SDS-PAGE technique. The results showed that: 8 HMW-GS alleles and 11 subunits combination were detected; 2 types of subunits were on locus Glu-A1, subunit 1 (83.67%) was the major subunit; 4 types of subunits were on locus Glu-B1, subunit 14+15 (42.86%) was the major subunit; subunit 2+12 (71.43%) and 5+10 (28.57%) were detected on locus Glu-D1; we determined the quality of the 49 wheat strains and conducted the correlation analysis between the quality and the subunits; the determination result of subunit 1 on locus Glu-A1 was higher than that of subunit Null; on locus Glu-D1, the quality of subunit 5+10 was higher than that of subunit 2+12; on locus Glu-B1, there were significant differences between the 4 types of subunits in qualities; subunit 7+8 had the highest contribution to the protein content, bulk density and wet gluten content; subunit 17+18 had the highest contribution to Zeleny sedimentation and maximum anti-extension resistance; subunit 7+9 had the longest stability time.

Key words: wheat, HMW-GS, quality, protein content, bulk density, stability time

中图分类号:

S512.1

王倩, 任文斌, 邱玉亮, 许琦, 谢三刚. 49份山西水地小麦品系的HMW-GS组成及品质分析[J]. 中国农学通报, 2020, 36(14): 11-15.

Wang Qian, Ren Wenbin, Qiu Yuliang, Xu Qi, Xie Sangang. 49 Wheat Strains in Shanxi: High-Molecular-Weight Glutenin Subunits (HMW-GS) Composition and Quality Analysis[J]. Chinese Agricultural Science Bulletin, 2020, 36(14): 11-15.

图/表 6

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品质指标	蛋白质	面筋	容重	沉淀值	最大抗延阻力	稳定时间
变异幅度	12.97%~18.64%	29.38%~39.86%	772~827 g/L	18.9~36.1 mL	150~484 BU	2.9~7.3 min
平均值	15.83%	34.58%	811 g/L	26.62 mL	237 BU	4.18 min
标准差	1.13%	2.14%	11 g/L	3.55 mL	60 BU	0.93 min
变异系数	0.07	0.062	0.014	0.13	0.25	0.22

品质指标	蛋白质	面筋	容重	沉淀值	最大抗延阻力	稳定时间
变异幅度	12.97%~18.64%	29.38%~39.86%	772~827 g/L	18.9~36.1 mL	150~484 BU	2.9~7.3 min
平均值	15.83%	34.58%	811 g/L	26.62 mL	237 BU	4.18 min
标准差	1.13%	2.14%	11 g/L	3.55 mL	60 BU	0.93 min
变异系数	0.07	0.062	0.014	0.13	0.25	0.22

含不同优质亚基数材料	蛋白质含量/%	容重/(g/L)	湿面筋含量/%	沉淀值/mL	最大抗延阻力/BU	稳定时间/min
含3个优质亚基的材料	16.41±0.43a	812.33±1.90a	35.92±0.84a	29.21±1.52ab	252.89±16.27a	4.36±0.26ab
含2个优质亚基的材料	15.35±0.21ab	809.2±2.61b	33.88±0.39b	26.06±0.63b	241.52±14.79b	4.32±0.21a
含1个优质亚基的材料	16.29±0.27b	816.00±1.73c	35.16±0.61c	25.48±0.70a	208.38±8.5c	3.66±0.08b

含不同优质亚基数材料	蛋白质含量/%	容重/(g/L)	湿面筋含量/%	沉淀值/mL	最大抗延阻力/BU	稳定时间/min
含3个优质亚基的材料	16.41±0.43a	812.33±1.90a	35.92±0.84a	29.21±1.52ab	252.89±16.27a	4.36±0.26ab
含2个优质亚基的材料	15.35±0.21ab	809.2±2.61b	33.88±0.39b	26.06±0.63b	241.52±14.79b	4.32±0.21a
含1个优质亚基的材料	16.29±0.27b	816.00±1.73c	35.16±0.61c	25.48±0.70a	208.38±8.5c	3.66±0.08b

变异位点	亚基	蛋白质含量/%	容重/(g/L)	湿面筋含量/%	沉淀值/ mL	最大抗延阻力/BU	稳定时间/min
Glu-A1	1	15.56±0.18	811.46±1.73	34.61±0.32	26.82±0.54	239.17±9.82	4.22±0.14
Glu-A1	null	15.53±0.47	807.88±4.97	34.06±1.05	25.85±1.48	226.50±22.16	4.04±0.40
Glu-B1	7+8	16.33±0.21a	813.79±3.26a	35.17±0.38a	27.04±0.74a	227.64±11.16a	4.01±0.23a
	7+9	16.09±0.40c	813.78±2.23b	34.75±0.89b	26.28±1.60b	218.11±18.26b	4.40±0.27b
	14+15	15.47±0.96a	808.76±2.67c	34.26±0.38c	25.85±0.62c	237.24±14.45c	4.26±0.20c
	17+18	15.79±1.07b	812.00±3.14d	34.36±2.06d	29.28±2.83d	264.00±25.42d	3.91±0.29d
Glu-D1	2+12	15.66±0.19	809.77±2.19	34.37±0.36	28.24±1.16	235.48±11.55	4.19±0.18
Glu-D1	5+10	16.17±0.33	813.64±1.72	34.90±0.64	26.03±0.51	241.14±12.36	4.20±0.19