Abstract: 【OBJECTIVE】To investigate the effect of diffrent fertilizers on the characteristics of carbon and nitrogen metabolization in Houttuynia cordata Thunb;【METHOD】A single factor randomized block design was employed to investigate net photosynthesis rate, the nitrate reductase activities, contents of Chla, Chlb，total chlorophyll and crude proteins in leaves of Houttuynia cordata.【RESULTS】The highest content of total chlorophyll was found in the treatment by 36000kg/hm2 human mature with a value of 2.552 mg/gFW. The highest net photosynthesis rate was found in all the treatments by mid-level fertilizers described above. The highest net photosynthesis rate was detected in the treatment by 36000kg/hm2 duck mature, with a value of 8.306μmol/(m2·s). Furthermore, the activities of nitrate reductase in leaves of Houttuynia cordata were in the order of mature of duck> mature of pig > conventional fertilizer >chemical fertilizer > mature of human from stronger to weaker. The nitrate reductase activities were the highest in the treatment by mature of pig at middle levels of fertilizer application with a value of 46.2μg NO2-/(h·g)FW. While the content of crude protein was the highest in the treatment by more higher level of chemical fertilizer(13.31%). 【CONCLUSION】The application of organic fertilizers could increas the contents of chlorophyll and net photosynthesis rate of Houttuynia cordata Thunb, and this was benefiacal to the accumulation of carbonhydrates in the plants. The application of mature of man might not profit the activities of nitrate reductase and the accumulation of protein.