转录因子ABP9基因过表达对植物生长发育的影响分析

摘要/Abstract

摘要： 【研究目的】为分析玉米ABA/逆境响应转录因子ABP9基因过量表达对植物生长发育的影响以利用该基因开展抗逆分子育种。【方法】本研究构建了35S启动子驱动ABP9组成型表达的植物表达载体，获得了过表达ABP9基因的拟南芥转基因株系，并在正常生长条件下，比较了转基因植株和野生型在种子萌发、营养生长和生殖生长阶段的形态和生理变化，分析了可能的分子机制。【结果】结果表明，正常生长条件下，转基因植株与野生型相比表现出萌发，幼苗生长以及开花阶段的生长抑制；气孔开度减小，叶片内源ABA含量降低；ABA信号传导基因表达增强，而ABA合成基因表达降低；而且这些效应在ABP9基因表达相对较强的转基因株系中表现更明显。【结论】说明ABP9基因过量在正常生长条件下即诱导转基因植株产生耐逆反应，抑制植株的生长发育。因此，在利用ABP9基因进行转基因抗逆育种时须考虑控制ABP9基因适时适量表达。

关键词: 高大韧稻, 高大韧稻, 特大穗, 恢复系, 超高产, 杂种优势

Abstract: 【OBJECTIVE】 In order to analyze the effects on plant growth and development of over-expressing ABP9, an ABA/stress-responsive transcription factor gene from maize, aiming at genetic engineering crops for abiotic stress tolerance. 【METHOD】 An plant expression vector on which the expression of ABP9 is driven by 35S promoter was constructed and transgenic Arabidopsis lines over-expressing ABP9 were obtained. The morphological, developmental, physiological and gene expression changes in transgenic plants comparing to wild type were analyzed at germination, vegetative and reproductive growth stages. 【RESULTS】 The results showed that under normal growth conditions, comparing to that of wild type plants, the growth and development of transgenic plants was delayed at germination, seedling growth and flowering stages. The stomata pores and leaf ABA levels reduced. The expression of genes involved in ABA signaling increased whereas that of ABA biosynthetic genes decreased. All of the effects are positively correlated with the expression levels of ABP9. 【CONCLUSION】 The data indicates that ABP9 over-expression induces stress responses in transgenic plants even under normal growth conditions and retards growth and development. This suggests that the timing and levels of ABP9 expression should be tightly controlled when using it to improve plant stress tolerance.

中图分类号:

孟慧,,张霞,曾日中,范云六,赵军. 转录因子ABP9基因过表达对植物生长发育的影响分析[J]. 中国农学通报, 2007, 23(6): 94-94.

Meng Hui,, Zhang Xia, Zeng Rizhong, Fan Yunliu, Zhao Jun. Effects of Transcription Factor ABP9 Over-expression on Plant Growth and Development[J]. Chinese Agricultural Science Bulletin, 2007, 23(6): 94-94.

参考文献

[1] Schroeder J I, Kwak J M, Allen G J, et al. Guand cell abscisic acid signaling and engineering drought hardiness in plants. Nature, 2001,410:327-330.
[2] Thomashow M F. Plant cold acclimation: freezing tolerance genes and regulatory mechanisms. Annu Rev Plant Physiol Plant Mol Biol, 1999,50:571-599.
[3] Xiong L, Schumaker K S, Zhu J K. Cell signaling during cold, drought, and salt stress Plant Cell, 2002,14(Suppl):S165-83.
[4] Giraudat J, Parcy F, Bertauche N, et al. Current advances in abscisic acid action and signaling. Plant Mol Bio, 1994,26:1557-1577.
[5] Busk P K and Pages M. Regulation of abscisic acid induced transcription. Plant Mol Bio, 1998,37:425-435.
[6] Finkelstein R R, Gampala S S, Rock C D. Abscisic acid signaling in seeds and seedlings. Plant Cell, 2002,14(Suppl):15-45.
[7] Leung J and Giraudat J. Abscisic acid signal transduction. Annu Rev Plant Physiol Plant Mol Biol, 1998,49,199-222.
[8] Zeevaart J A D and Creelman R A. Metabolism and physiology of abscisic acid. Annu Rev Plant Physiol Plant Mol Biol, 1988,39:439-473.
[9] Nambara E, Marion-Poll A. ABA action and interactions in seeds. Trends Plant Sci, 2003,8(5):213-217.
[10] Mishra G, Zhang W, Deng F, et al. A bifurcating pathway directs abscisic acid effects on stomatal closure and opening in Arabidopsis. Science, 2006,312(5771):264-266.
[11] Assmann S M. OPEN STOMATA1 opens the door to ABA signaling in Arabidopsis guard cells.Trends Plant Sci, 2003,8(4):151-153.
[12] Schroeder J I, Kuhn J M. Plant biology: abscisic acid in bloom. Nature, 2006,439(7074):277-278.
[13] Razem F A, El-Kereamy A, Abrams S R, et al. The RNA-binding protein FCA is an abscisic acid receptor. NATURE, 2006,439(7074):290-294.
[14] Jiang M and Zhang J. Effect of abscisic acid on active oxygen species, antioxidative defence system and oxidative damage in leaves of maize seedlings. Plant and Cell Physiology, 2001,42:1265-1273.
[15] Himmelbach A, Iten M, Grill E, et al. Signaling of abscisic acid to regulate plant growth. Philos Trans R Soc Lond B, 1998,353:1439-1444.
[16] Uno Y, Furihata T, Abe H, et al. Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependant signal transduction pathway under drought and high-salinity. Proc. Natl. Acad. Sci. USA, 2000,97:11632-11637.
[17] Choi H, Hong J, Ha J, et al. ABFs, a family of ABA-responsive element binding factors. J Biol Chem, 2000,21:1723-1730.
[18] Kang J, Choi H, Im M, et al. Arabidopsis basic leucine zipper proteins that mediate stress-responsive abscisic acid signaling. Plant Cell, 2002,14:343-357
[19] 王磊,赵军,范云六.玉米Cat1基因顺式元件ABRE2结合蛋白ABP9的基因克隆及功能分析.科学通报,2002,47(15):1167-1171.
[20] Bechtold N, and Pelletier G. In planta Agrobacterium mediated transformation of adult Arabidopsis thaliana plants by vacuum infiltration. Methods Mol Biol, 1998,82:259-266.
[21] Pei Z M, Murata Y, Schroeder J I, et al. Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells. Nature, 2000,406(6797):731-734.
[22] Kwak J M, Murata Y, Schroeder J I, et al. Dominant negative guard cell K+ channel mutants reduce inward-rectifying K+ currents and light-induced stomatal opening in Arabidopsis. Plant Physiol1, 2001,27(2):473-485.
[23] Weiler E W. An enzyme immunoassay for Cis(+) abscisic acid. Physiol. Plant, 1982,54:510-514.
[24] Hsiao T C. Plant responses to water stress. Annual Review of Plant Physiology, 1973,24:519-570.