Abstract: Application of N fertilizer and water are the most important measures that increase grain yield of wheat. The field experiment was carried out to study the effect of nitrogen application on grain yield of winter wheat, efficiency of nitrogen and NO3--N accumulation in soil at Wuqiao Experimental Station of China Agricultural University. A randomized complete block design was used with three replications with the cultivar Jinan17 in two irrigation modes. There were four nitrogen rates(0、157.5kg/hm2、226.5kg/hm2、295.5kg/hm2) and two methods(basal nitrogen application and split nitrogen application) within seven treatments. The results showed that the grain yield of winter wheat with two-irrigation was higher than that with one-irrigation with the same nitrogen application rate and method. Compared to one-irrigation，the grain number per spike in two-irrigation was higher，the 1000-grain weight was lower, the water wastage was higher, but the WUE was lower. The yield was not affected significantly by nitrogen rates and the methods. Within the nitrogen rate of 157.5-295.5kg/hm2， the lower NUPE and Nitrogen Productivity declined with nitrogen rate increasing. NHI was not lower than the others at the lowest nitrogen rate of 157.5kg/hm2. The accumulation of NO3--N in soil was significantly and positively correlated with the nitrogen application rate. NO3--N in one-irrigation was distributed mainly in the top layer (0-80cm), but it was inverse in two-irrigation. It was concluded that it is the most reasonable cultivation mode in water-limited area to apply one-irrigation，nitrogen application rate of 157.5kg/hm2 as basal fertilizer，which not only increased the nitrogen and water utilization efficiency，but also deceased the leach and the accumulation of NO3--N in soil.