Abstract: Effects of pressure, temperature, time and dosage of chemical preparation in supercritical CO2 technology on extract abilities of flavone from Lonicera japonica Thunb were Piloted in this paper. And the yield of the flavonids was determined by UV spectrophotometer . This paper also compared the method supercritical CO2 extraction with hot alcohol extraction, soaking microwave assisted extraction and ultrasonic assisted extraction .Results showed that following conditions were optimal: pressure 30.0MPa, temperature 40℃, time 120min and dosage of chemical preparation 4.5ml/g. Extraction yield of hot alcohol ,soaking microwave assisted extraction ,ultrasonic assisted extraction and supercritical CO2 technology extraction were 8.92%、9.56% 、9.32% and 10.24% separately, and supercritical CO2 technology was a good extraction method for its high yield and short times.