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中国农学通报

中国农学通报 ›› 2007, Vol. 23 ›› Issue (12): 239-239.

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低pH对葛藤根瘤菌生长的影响

魏世清,张 磊,张学军,石 洁   

  • 出版日期:2007-12-05 发布日期:2007-12-05

The effect of low pH on growth of rhizobium of Pueraria lobata

Wei Shiqing, Zhang Lei, Zhang Xuejun, Shi Jie   

  • Online:2007-12-05 Published:2007-12-05

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摘要： 为了研究低pH对葛藤(Pueraria lobata)根瘤菌PR390生长的影响，选取葛藤根瘤菌PR390作为研究对象，设置pH7.0、5.5、5.0、4.8、4.6、4.4六个梯度对根瘤菌生长的影响；研究结果表明：低pH对PR390菌体、菌落大小产生了明显抑制作用；在低pH的液体培养基中PR390表现为生长延滞期增长，对数生长期滞后，稳定期延迟，平均代时增长；在不同酸性土壤悬液中，土壤悬液酸性降低，根瘤菌数目随之减少。说明低pH对PR390生长产生了明显抑制作用，同时表明PR390具有较强的耐酸能力，能够抵制低pH对菌体产生的危害。

关键词: 大蒜, 大蒜, 体细胞胚, 组织学研究

Abstract: The effect of low pH on growth of rhizobium of Pueraria lobata was studied in this paper. PR390 was studied in this trail and the effect of low pH on growth of PR390 was investigated. The results indicated that low pH obviously inhibited the thalli and colony of PR390. The lower the acidity was, the slower the growth of rhizobia with the characteristics of a longer lag phase, a lagged log phase, a delayed stationary phase and a longer generation time were. The number of rhizobia decreased with the pH value of soil reducing. Furthermore, the results indicated PR390 had strong acid-tolerant ability and showed resistance to low pH.

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