Abstract: To study the effects of different base bio-organic fertilizer on output and quality of Houttuynia cordata Thunb(HCT) grown in substrates; Eco-organic type soilless culture was empolyed, the root weight and the leaf weight, contents of Vc, total sugar, protein and mineral elements were tested; The highest yield and profit occurred at the treatment of 16kg bio-organic fertilizer per cubic meter substrates. There was negative relationship between base fertilizer rate and HCT Vc content. Potassium content in root and leaf, phosphorus content in leaf increased, while sodium content in root and leaf and magnesium content in leaf decreased with the base fertilizer rate increasing; It was recommended that optimum rate of base fertilizer for better yield, quality and profit in HCT production was 16kg bio-organic fertilizer per cubic meter substrates.