Abstract: The composition of fermented broth and fermentation conditions were optimized by monofactorial and orthogonal experiment. Combination and content of the C and N sources, as well as mental ions, have different effect on the growth and antagonistic effect of bacterium CE. The broth composed of 4.5% corn flour and 0.15% yeast extract is better for both growth and antagonistic effect of CE. Adding of Fe3+ can promote both growth and antagonistic effect. The antagonistic effect of bacterium CE is correlation with growth under the studied conditions in a certain extent, that is, the antagonistic effect is increased with the growth of CE. The most preferable condition is 30℃, pH7, 140r/min, 10% inoculation, and fermented for 60h.