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中国农学通报

中国农学通报 ›› 2008, Vol. 24 ›› Issue (11): 78-80.

所属专题： 生物技术； 小麦

• 生物技术科学 • 上一篇    下一篇

小麦甲基结合蛋白基因MBD3重组表达载体的构建和原核表达

孟凡荣,司志飞,刘昊英,张问   

  • 收稿日期:2008-08-12 修回日期:2008-09-08 出版日期:2008-11-05 发布日期:2008-11-05

Recombinant expression vector with wheat MBD3 gene and its prokaryotic expression

Meng Fanrong, Si Zhifei, Liu Haoying, Zhang Wen   

  • Received:2008-08-12 Revised:2008-09-08 Online:2008-11-05 Published:2008-11-05

PDF (PC)

860

摘要： 本研究构建了小麦甲基结合蛋白基因MBD3的原核表达载体pGEX-4T-MBD3, 转化大肠杆菌BL21 (DE3)工程菌株，在37 ºC，1 mM IPTG浓度条件下，成功诱导表达了的GST-MBD3融合蛋白，大小为49.6 kDа，这为进一步开展MBD3的蛋白纯化和功能分析奠定了基础。

关键词: 肉牛, 肉牛, 三元杂交, 增重

Abstract: In this study, a prokaryotic expression vector pGEX-4T-MBD3 for the wheat MBD (Methyl-binding domain protein) gene MBD3 was constructed. Transform it to E. coli. BL21 (DE3), the fusion protein GST-MBD3 with molecular weight of 49.6 kDа was effectively expressed under 1mM IPTG concentrations at 37°C, which provides a foundation for further purification and functional study of the MBD3 protein.